Light controls mesophyll- specific posttranscriptional splicing of photoregulatory genes by AtPRMT5

文献类型： 外文期刊

第一作者： Yan, Yan

作者： Yan, Yan;Luo, Haofei;Hou, Yifeng;Deng, Xian;Cao, Xiaofeng;Yan, Yan;Luo, Haofei;Hou, Yifeng;Deng, Xian;Cao, Xiaofeng;Qin, Yuwei;Jia, Jinbu;Liu, Zhijian;Zhai, Jixian;Long, Yanping;Yan, Tingting;Cao, Xiaofeng

作者机构：

关键词： post transcriptional splicing; single- nucleus RNA profiling; mesophyll cell; AtPRMT5; COP1

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.1； 五年影响因子：12.0 ）

ISSN： 0027-8424

年卷期： 2024 年 121 卷 6 期

页码：

收录情况： SCI

摘要： Light plays a central role in plant growth and development, providing an energy source and governing various aspects of plant morphology. Previous study showed that many polyadenylated full- length RNA molecules within the nucleus contain unspliced introns (post- transcriptionally spliced introns, PTS introns), which may play a role in rapidly responding to changes in environmental signals. However, the mechanism underlying post- transcriptional regulation during initial light exposure of young, etiolated seedlings remains elusive. In this study, we used FLEP-seq2, a Nanopore-based sequencing technique, to analyze nuclear RNAs in Arabidopsis (Arabidopsis thaliana) seedlings under different light conditions and found numerous light- responsive PTS introns. We also used single- nucleus RNA sequencing (snRNA-seq) to profile transcripts in single nucleus and investigate the distribution of light- responsive PTS introns across distinct cell types. We established that light- induced PTS introns are predominant in mesophyll cells during seedling de- etiolation following exposure of etiolated seedlings to light. We further demonstrated the involvement of the splicing- related factor A. thaliana PROTEIN ARGININE METHYLTRANSFERASE 5 (AtPRMT5), working in concert with the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), a critical repressor of light signaling pathways. We showed that these two proteins orchestrate light- induced PTS events in mesophyll cells and facilitate chloroplast development, photosynthesis, and morphogenesis in response to ever- changing light conditions. These findings provide crucial insights into the intricate mechanisms underlying plant acclimation to light at the cell - type level.

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