A fluorescein-based "turn-on" fluorescence probe for hypochlorous acid detection and its application in cell imaging

文献类型： 外文期刊

第一作者： Li, Jie

作者： Li, Jie;Yang, Xiaopeng;Liu, Yongxiang;Tang, Jun;Zhao, Yufen;Ye, Yong;Zhang, Di;Zhao, Yufen;Ye, Yong;Li, Yan

作者机构：

关键词： Fluorescence probe; Fluorescein; C=N isomerization; HC1O; Cell imaging

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN： 0925-4005

年卷期： 2018 年 265 卷

页码：

收录情况： SCI

摘要： A simple fluorescent probe F-BH was designed and synthesized, introducing C=N group which can be a recognition site for hypochlorous acid into the fluorescent probe molecule by using 2-benzothiazole hydrazone and formylated fluorescein. The fluorescence intensity of the probe F-BH solution was very weak, while the fluorescence of the solution was obviously enhanced and the color of the solution changed after the addition of C10(-) ion. The selection and competition experiments proved that the probe has great selectivity for recognition of hypochlorous acid over other ROSs/RNSs and anions. Fluorescence titration experiments show that the probe has a low detection limit (17.5 nM). The probe has been successfully applied to real-time detection of hypochlorous acid in Hela cells. (C) 2018 Elsevier B.V. All rights reserved.

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