Simultaneous saccharification and co-fermentation of steam-exploded poplar wood to ethanol with Escherichia coli KO11

文献类型： 外文期刊

第一作者： Ai, B. L.

作者： Ai, B. L.;Sheng, Z. W.;Zheng, L. L.;Zheng, X. Y.;Wang, Q. Y.;Wang, Y. Q.

作者机构：

关键词： Steam explosion pretreatment; Simultaneous saccharification and co-fermentation; Poplar wood; Ethanol

期刊名称：BULGARIAN CHEMICAL COMMUNICATIONS （ 影响因子：0.242； 五年影响因子：0.322 ）

ISSN： 0324-1130

年卷期： 2017 年 49 卷

页码：

收录情况： SCI

摘要： Steam explosion pretreatment is a promising method for the preparation of lignocellulosic biomass for biofuels production. Steam explosion was performed to assess the effectiveness of pretreating poplar wood for ethanol production. Maintaining steam pressure at 4 MPa for 10 min, 66.8% of hemicellulose and 5.3% of cellulose were hydrolyzed. The scanning electron microscopy observation, FT-IR spectroscopy analysis and X-ray diffraction analysis showed that hydrolysis of hemicellulose and modification of cellulose and lignin structures improved the accessibility of cellulose to hydrolytic enzymes. Cellulase production by Trichoderma reesei RUT C-30 was initiated with inocula prepared by different methods. It was found that the composition of pre-culture medium and inoculum age have minimal or no impact on cellulase production. Using the steam-exploded poplar wood as feedstock with cellulase loading of 20 FPIU g(-1) substrate, ethanol production by gene-engineered Escherichia coli KO11 reached 3.6 g L-1, corresponding to 51.4% of the maximum theoretical yield based on glucan remaining in the input substrate. The ethanol yield was limited by the low solid content of steam-exploded residue, and inhibited by the inhibitors released in the pretreatment process. So the steam explosion process needs to be improved for lower water content and less inhibitors, and detoxification is necessary to achieve a good fermentability of pretreated poplar wood to ethanol.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序