Insights Into the Roles of Two Genes of the Histidine Biosynthesis Operon in Pathogenicity of & IT;Xanthomonas & IT; & IT;oryzae & IT;pv. & IT;oryzicola & IT;

文献类型： 外文期刊

第一作者： Su, Panpan

作者： Su, Panpan;Song, Zhiwei;Zhao, Yancun;Wu, Guichun;Zhang, Yuqiang;Wang, Bo;Qian, Guoliang;Lin, Fengquan;Wu, Guichun;Zhang, Yuqiang;Wang, Bo;Qian, Guoliang;Lin, Fengquan;Fu, Zheng Qing

作者机构：

期刊名称：PHYTOPATHOLOGY （ 影响因子：4.025； 五年影响因子：4.394 ）

ISSN： 0031-949X

年卷期： 2018 年 108 卷 5 期

页码：

收录情况： SCI

摘要： Xanthomonas oryzae pv. oryzicola is an X. oryzae pathovar that causes bacterial leaf streak in rice. In this study, we performed functional characterization of a nine-gene his operon in X. oryzae pv. oryzicola. Sequence analysis indicates that this operon is highly conserved in Xanthomonas spp. Auxotrophic assays confirmed that the his operon was involved in histidine biosynthesis. We found that two genes within this operon, trpR and hisB, were required for virulence and bacterial growth in planta. Further research revealed that trpR and hisB play different roles in X. oryzae pv. oryzicola. The trpR acts as a transcriptional repressor and could negatively regulate the expression of hisG, -D, -C, -B, -H, -A, and -E hisB, which encodes a bifunctional enzyme implicated in histidine biosynthesis, was shown to be required for xanthomonadin production in X. oryzae pv. oryzicola. The disruption of hisB reduced the transcriptional expression of five known shikimate pathway-related genes xanB2, aroE, aroA, aroC, and aroK. We found that the his operon in X. oryzae pv. oryzicola is not involved in hypersensitive response in nonhost tobacco plants. Collectively, our results revealed that two genes in histidine biosynthesis operon play an important role in the pathogenicity of X. oryzae pv. oryzicola Rs105.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序