A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

文献类型： 外文期刊

第一作者： Sun, Yaru

作者： Sun, Yaru;Cheng, Yuening;Lin, Peng;Yi, Li;Tong, Mingwei;Cao, Zhigang;Wang, Gaili;Li, Shuang;Cheng, Shipeng;Wang, Jianke;Sun, Yaru;Cheng, Yuening;Lin, Peng;Yi, Li;Tong, Mingwei;Cao, Zhigang;Wang, Gaili;Li, Shuang;Cheng, Shipeng;Wang, Jianke;Yuan, Wanzhe

作者机构：

关键词： Canine parvovirus; Multiplex real-time PCR; Detection; Differentiation; Antigenic types

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2018 年 38 卷

页码：

收录情况： SCI

摘要： Canine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), was able to differentiate CPV-2 and its variants (CPV-2a, 2b and 2c). Another set of primers and probes, CPV-426F/CPV-426R and CPV-2426P (for CPV-2 and 2a)/CPV-2b-426P (for CPV-2b)/CPV-2c-426P (for CPV 2c), was able to differentiate CPV-2a (2), CPV-2b, and CPV-2c. With these primers and probes, the multiplex TaqMan real-time PCR assay detected effectively and differentiated CPV-2, 2a, 2b and 2c by two separate real-time PCRs. No cross reactivity was observed with canine distemper virus, canine adenovirus, and canine coronavirus. The detection limit of the assay is 10(1) genome copies/pL for CPV-2, CPV-2a, CPV-2b, and 10(2) copies/pL for CPV-2c. The multiplex realtime PCR has 100% agreement with DNA sequencing. We provide a sensitive assay that simultaneously detects and differentiate four antigenic types of CPV and the method was also used for quantification of CPVs viral genome.

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