Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method
文献类型： 外文期刊
第一作者： Fei, Dongliang
作者： Fei, Dongliang;Wei, Dong;Yu, Xiaolei;Yue, Jinjin;Li, Ming;Sun, Li;Jiang, Lili;Ma, Mingxiao;Fei, Dongliang;Li, Yijing;Diao, Qingyun
作者机构：
关键词： CSBV; Capsid protein VP3; Yeast two-hybrid; Protein interaction; Galectin
期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）
ISSN： 0168-1702
年卷期： 2018 年 248 卷
页码：
收录情况： SCI
摘要： Chinese sacbrood virus (CSBV) causes larval death and apiary collapse of Apis cerana. VP3 is a capsid protein of CSBV but its function is poorly understood. To determine the function of VP3 and screen for novel binding proteins that interact with VP3, we conducted yeast two-hybrid screening, glutathione S-transferase pull-down, and co-immunoprecipitation assays. Galectin (GAL) is a protein involved in immune regulation and host-pathogen interactions. The yeast two-hybrid screen implicated GAL as a major VP3-binding candidate. The assays showed that the VP3 interacted with GAL. Identification of these cellular targets and clarifying their contributions to the host-pathogen interaction may be useful for the development of novel therapeutic and prevention strategies against CSBV infection.
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