Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method

文献类型： 外文期刊

第一作者： Fei, Dongliang

作者： Fei, Dongliang;Wei, Dong;Yu, Xiaolei;Yue, Jinjin;Li, Ming;Sun, Li;Jiang, Lili;Ma, Mingxiao;Fei, Dongliang;Li, Yijing;Diao, Qingyun

作者机构：

关键词： CSBV; Capsid protein VP3; Yeast two-hybrid; Protein interaction; Galectin

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2018 年 248 卷

页码：

收录情况： SCI

摘要： Chinese sacbrood virus (CSBV) causes larval death and apiary collapse of Apis cerana. VP3 is a capsid protein of CSBV but its function is poorly understood. To determine the function of VP3 and screen for novel binding proteins that interact with VP3, we conducted yeast two-hybrid screening, glutathione S-transferase pull-down, and co-immunoprecipitation assays. Galectin (GAL) is a protein involved in immune regulation and host-pathogen interactions. The yeast two-hybrid screen implicated GAL as a major VP3-binding candidate. The assays showed that the VP3 interacted with GAL. Identification of these cellular targets and clarifying their contributions to the host-pathogen interaction may be useful for the development of novel therapeutic and prevention strategies against CSBV infection.

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