Induction of apoptosis in human cervical carcinoma HeLa cells by active compounds from Hypericum ascyron L.

文献类型： 外文期刊

第一作者： Li, Xiu-Mei

作者： Li, Xiu-Mei;Yang, Pei-Long;Luo, Xue-Gang;He, Jun-Fang;Wang, Nan;Zhou, Hao;Zhang, Tong-Cun;Zhang, Tong-Cun

作者机构：

关键词： Hypericum ascyron L.; anti-proliferative agent; activity compound; human cervical carcinoma cell; apoptosis

期刊名称：ONCOLOGY LETTERS （ 影响因子：2.967； 五年影响因子：2.575 ）

ISSN： 1792-1074

年卷期： 2018 年 15 卷 3 期

页码：

收录情况： SCI

摘要： Hypericum ascyron L. (Great St. Johnswort), which belongs to the Hypericaceae family, has been used for the treatment of hematemesis, metrorrhagia, rheumatism, swelling, stomach ache, abscesses, dysentery and irregular menstruation for >2,000 years in China. The aim of the present study was to clarify the anticancer activity compounds from H. ascyron L. and the underlying molecular mechanism. Anticancer activity of H. ascyron L. extract was evaluated using an MTT assay. To confirm the anticancer mechanism of activity compounds, Hoechst 33258, Annexin V-fluorescein isothiocyanate/propidium iodide, 2',7'-dichlorodihydro-fluorescein diacetate, rhodamine 123 staining and caspase-3 activity analysis were performed. The results demonstrated that the anti-proliferative action of the mixture of kaempferol 3-O-beta-(2"-acetyl) galactopyranoside (K) and quercetin (Q) (molar ratio, 1:1) was significantly increased compared with either of these two compounds separately, and the active fraction of the H. ascyron L. extract vertical bar(HALE). HALE, indicating that the anti-proliferative function of H. ascyron L. may be a synergic effect of K and Q. Furthermore, the inhibitory effect of KQ on the growth of HeLa cells was mediated by the induction of apoptosis. To the best of our knowledge, the present study is the first to identify that KQ exhibits significant anti-proliferation activity on HeLa cells via the apoptotic pathway, and is also the first to evaluate the anticancer potential of H. ascyron L. The results of the present study may provide a rational base for the use of H. ascyron L. in the clinic, and shed light on the development of novel anticancer drugs.

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