The Fusarium graminearum Histone Acetyltransferases Are Important for Morphogenesis, DON Biosynthesis, and Pathogenicity

文献类型： 外文期刊

第一作者： Xu, Jingsheng

作者： Xu, Jingsheng;Xu, Jin;Zhang, Hao;Chen, Wanquan;Feng, Jie;van Diepeningen, Anne D.;van der Lee, Theo A. J.;Waalwijk, Cees

作者机构：

关键词： Fusarium graminearum; histone acetyltransferase; secondary metabolism; deoxynivalenol; pathogenicity

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Post-translational modifications of chromatin structure by histone acetyltransferase (HATs) play a central role in the regulation of gene expression and various biological processes in eukaryotes. Although HAT genes have been studied in many fungi, few of them have been functionally characterized. In this study, we identified and characterized four putative HATs (FgGCN5, FgRTT109, FgSAS2, FgSAS3) in the plant pathogenic ascomycete Fusarium graminearum, the causal agent of Fusarium head blight of wheat and barley. We replaced the genes and all mutant strains showed reduced growth of F graminearum. The Delta FgSAS3 and Delta FgGCN5 mutant increased sensitivity to oxidative and osmotic stresses. Additionally. Delta FgSAS3 showed reduced conidia sporulation and perithecium formation. Mutant Delta FgGCN5 was unable to generate any conidia and lost its ability to form perithecia. Our data showed also that FgSAS3 and FgGCN5 are pathogenicity factors required for infecting wheat heads as well as tomato fruits. Importantly, almost no Deoxynivalenol (DON) was produced either in Delta FgSAS3 or Delta FgGCN5 mutants, which was consistent with a significant downregulation of TRI genes expression. Furthermore, we discovered for the first time that FgSAS3 is indispensable for the acetylation of histone site H3K4, while FgGCN5 is essential for the acetylation of H3K9, H3K18, and H3K27. H3K14 can be completely acetylated when FgSAS3 and FgGCN5 were both present. The RNA-seq analyses of the two mutant strains provide insight into their functions in development and metabolism. Results from this study clarify the functional divergence of HATs in F graminearum, and may provide novel targeted strategies to control secondary metabolite expression and infections of F graminearum.

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