A Golden Gate and Gateway double-compatible vector system for high throughput functional analysis of genes

文献类型： 外文期刊

第一作者： Luo, Yanjie

作者： Luo, Yanjie;Qiu, Yang;Meerja, Farida;Lu, Qing Shi;Tian, Lining;Qiu, Yang;Na, Ren;Yang, Chunyan;Meerja, Farida

作者机构：

关键词： Golden Gate assembly; Gateway cloning; BiFC; Artificial microRNA; CRISPR/Cas9

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2018 年 271 卷

页码：

收录情况： SCI

摘要： A major research topic nowadays is to study and understand the functions of the increasing number of predicted genes that have been discovered through the complete genome sequencing of many plant species. With the aim of developing tools for rapid and convenient gene function analysis, we have developed a set of "pGate" vectors based on the principle of Golden gate and Gateway cloning approaches. These vectors combine the positive aspects of both Golden gate and Gateway cloning strategies. pGate vectors can not only be used as Golden gate recipient vectors to assemble multiple DNA fragments in a pre-defined order, but they can also work as an entry vector to transfer the assembled DNA fragment(s) to a large number of already-existing, functionally diverse, Gateway compatible destination vectors without adding additional nucleotides during cloning. We show the pGate vectors are effective and convenient in several major aspects of gene function analyses, including BiFC (Bimolecular fluorescence complementation) to analyze protein-protein interaction, amiRNA (artificial microRNA) candidate screening and as assembly of CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats, CRISPR-associated protein-9 nuclease) system elements together for genome editing. The pGate system is a practical and flexible tool which can facilitate plant gene function research.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序