Quantitative phosphoproteomic analysis of caprine muscle with high and low meat quality

文献类型： 外文期刊

第一作者： Liu, Manshun

作者： Liu, Manshun;Wei, Yanchao;Zhao, Jing;Zhong, Huazhen;Liu, Yongfeng;Li, Xin;Zhang, Dequan;Quek, Siew Young

作者机构：

关键词： Quantitative phosphoproteomic; Caprine muscle; Meat quality; Rigor mortis

期刊名称：MEAT SCIENCE （ 影响因子：5.209； 五年影响因子：5.305 ）

ISSN： 0309-1740

年卷期： 2018 年 141 卷

页码：

收录情况： SCI

摘要： During the conversion of muscle to meat, protein phosphorylation can regulate various biological processes that have important effects on meat quality. To investigate the phosphorylation pattern of protein on rigor mortis, goat longissimus thoracis and external intercostals were classified into two groups (high quality and low quality), and meat quality was evaluated according to meat quality attributes (Warner-Bratzler shear force, Color, pH and drip loss). A quantitative mass spectrometry-based phosphoproteomic study was conducted to analyze the caprine muscle at 12 h postmortem applying the TiO2-SIMAC-HILIC (TiSH) phosphopeptide enrichment strategy. A total of 2125 phosphopeptides were identified from 750 phosphoproteins. Among them, 96 proteins had differed in phosphorylation levels. The majority of these proteins are involved in glucose metabolism and muscle contraction. The differential phosphorylation level of proteins (PFK, MYL2 and HSP27) in two groups may be the crucial factors of regulating muscle rigor mortis. This study provides a comprehensive view for the phosphorylation status of caprine muscle at rigor mortis, it also gives a better understanding of the regulation of protein phosphorylation on various biological processes that affect the final meat quality attributes.

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