Isolation, Transformation and Overexpression of Sugarcane SoP5CS Gene for Drought Tolerance Improvement

文献类型： 外文期刊

第一作者： Thi-Thu Phan

作者： Thi-Thu Phan;Xing, Yong-Xiu;Yang, Li-Tao;Li, Yang-Rui

作者机构：

关键词： Sugarcane; P5CS gene; Drought tolerance; Overexpression; Transformation

期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）

ISSN： 0972-1525

年卷期： 2018 年 20 卷 4 期

页码：

收录情况： SCI

摘要： Drought is one of the very important growth-limiting factors for sugarcane production in China. Delta(1)-pyrroline-5-carboxylate synthase (P5CS) is the rate-limiting enzyme in proline synthesis, and it plays an important role in plant response to drought stress. In this study, sugarcane P5CS (SoP5CS) gene was cloned and submitted in GenBank with the accession number KJ546350. The gene SoP5CS was 2151 bp in length, encoding 716 amino acids with a predicted molecular weight of 77.73 kDa and an isoelectric point of 6.14. The qRT-PCR analysis showed that the expression of SoP5CS was higher in leaf than in root and stalk, and strongly induced under ABA, PEG, NaCl and 4 degrees C treatments. The plant excessive expression vector of SoP5CS was built and transformed into sugarcane calli by Agrobacterium-mediated transformation method, and transgenic sugarcane plants were obtained. Under drought stress, some transgenic lines with overexpressed SoP5CS showed significantly higher in SoP5CS expression level, proline accumulation, abscisic acid content, superoxide dismutase activity and relative water content but lower MDA content and chlorophyll (SPAD value) decrease as compared with the WT plants. Moreover, all the transgenic plants increased drought stress tolerance. Our findings indicated that SoP5CS plays an important role in response to abiotic stresses and overexpression of SoP5CS can improve drought tolerance in transgenic sugarcane plants.

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