Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera

文献类型: 外文期刊

第一作者: Deng, Yao

作者: Deng, Yao;Jia, Hao-Kang;Liu, Yu-Di;Hou, Mao-Lin;Miao, Yu-Tong;Deng, Yao;Jia, Hao-Kang;Liu, Yu-Di;Hou, Mao-Lin

作者机构:

期刊名称:PLOS ONE ( 影响因子:3.24; 五年影响因子:3.788 )

ISSN: 1932-6203

年卷期: 2018 年 13 卷 5 期

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收录情况: SCI

摘要: The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice.

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