microRNA profiling between Bacillus thuringiensis Cry1Ab-susceptible and -resistant European corn borer, Ostrinia nubilalis (Hubner)
文献类型: 外文期刊
第一作者: Li, X.
作者: Li, X.;Zhang, Q.;Zhou, X.;Li, X.;Coates, B. -S.;Zhang, Q.;Siegfried, B. -D.
作者机构:
关键词: European corn borer; Ostrinia nubilalis; miRNA; Cry1Ab; Bt resistance
期刊名称:INSECT MOLECULAR BIOLOGY ( 影响因子:3.585; 五年影响因子:3.215 )
ISSN: 0962-1075
年卷期: 2018 年 27 卷 3 期
页码:
收录情况: SCI
摘要: Transgenic maize hybrids that express insecticidal Bacillus thuringiensis (Bt) crystalline (Cry) protein toxins effectively protect against the European corn borer, Ostrinia nubilalis, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of O.nubilalis resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post-transcriptional gene regulation. Their potential roles in the evolution of Bt resistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab-susceptible and resistant O.nubilalis larvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up- and down-regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real-time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putative Bt-resistance genes aminopeptidase N and cadherin. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution of Bt resistance.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period
作者:Qi, J.;Li, J.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Hou, S.
关键词:Serum; biochemical indicator; laying period; GWAS; ducks
-
Phosphorylation promoted the glycolytic rate in postmortem meat by tightening the structure of glycogen phosphorylase
作者:Bai, Y.;Li, X.;Ren, C.;Zheng, X.;Hou, C.;Chen, L.;Zhang, D.
关键词:glycogen phosphorylase; protein phosphorylation; activity; protein structure simulation
-
Expression and self-assembly of virus-like particles from porcine parvovirus and its application in antibody detection
作者:Li, Y.;Yue, W.;Li, X.;Chen, Y.;Gao, Y.;Li, Y.;Yue, W.;Li, X.;Chen, Y.;Gao, Y.;Wang, Q.
关键词:porcine parvovirus; virus -like particles; indirect ELISA
-
Plant size, latitude, and phylogeny explain within-population variability in herbivory
作者:Robinson, M. L.;Cinto Mejia, E.;Cope, O. L.;Dole, H. E.;Kalwajtys, M.;Lyon, N. J.;Zehr, L. N.;Wetzel, W. C.;Robinson, M. L.;Hahn, P. G.;Calixto, E. S.;Inouye, B. D.;Underwood, N.;Whitehead, S. R.;Florjancic, G. A.;Abbott, K. C.;Bruna, E. M.;Bruna, E. M.;Cacho, N. I.;Dyer, L. A.;Forister, M. L.;Pringle, E. G.;Salcido, D. M.;Yim, S.;Abdala-Roberts, L.;Quijano, T.;Allen, W. J.;Maia, L. F.;Andrade, J. F.;Santos, B. A.;Angulo, D. F.;Anjos, D.;Anstett, D. N.;Wetzel, W. C.;Anstett, D. N.;Martin, B. S.;Bagchi, R.;LaScaleia, M. C.;Bagchi, S.;Barbosa, M.;Cornelissen, T.;Fernandes, G. W.;Maia, R. A.;Pereira, C. C.;Barrett, S.;Baskett, C. A.;Ben-Simchon, E.;Segre, H.;Shelef, O.;Ben-Simchon, E.;Segre, H.;Bloodworth, K. J.;Koerner, S. E.;Komatsu, K. J.;Bronstein, J. L.;Luizzi, V. J.;Buckley, Y. M.;Finn, A.;Puy, J.;Burghardt, K. T.;McGurrin, K.;Bustos-Segura, C.;Grof-Tisza, P.;Rasmann, S.;Carvalho, R. L.;Castagneyrol, B.;Chiuffo, M. C.;Cinoglu, D.;Greig, K. A.;Mijango-Ramos, Z.;Morrison, C. R.;Cock, M. C.;Cogni, R.;Cope, O. L.;Cortez, D. R.;Smith, D. S.;Crowder, D. W.;Dallstream, C.;Dattilo, W.;Davis, J. K.;Poveda, K.;Schroeder, H.;Dimarco, R. D.;Dimarco, R. D.;Egbon, I. N.;Ejomah, A.;Uyi, O.;Eisenring, M.;Gossner, M. M.;Elderd, B. D.;Kluse, J.;Endara, M. -J.;Eubanks, M. D.;Helms, A. M.;Everingham, S. E.;Mannall, T. L.;Everingham, S. E.;Mills, C. H.;Moles, A. T.;Farah, K. N.;Fox, Q. N.;Penczykowski, R. M.;Farias, R. P.;Fernandes, A. P.;Fernandes, G. W.;Ferrante, M.;Lamelas-Lopez, L.;Ferrante, M.;Frago, E.;Franca, F. M.;Maia, L. F.;Franca, F. M.;Getman-Pickering, Z.;Gianoli, E.;Gooden, B.;Gossner, M. M.;Gripenberg, S.;Jackson, E. E.;Groenteman, R.;Haack, N.;Hahn, L.;Haq, S. M.;Hennecke, J.;Hennecke, J.;Rosche, C.;Hermann, S. L.;Kersch-Becker, M. F.;Tooker, J. F.;Holeski, L. M.;Holeski, L. M.;Holm, S.;Valtonen, A.;Holm, S.;Hutchinson, M. C.;Kagiya, S.;Utsumi, S.;Kalske, A.;Karban, R.;Rosenheim, L. Y.;Rosenheim, J. A.;Kariyat, R.;Keasar, T.;Kharouba, H. M.;Kim, T. N.;Kimuyu, D. M.;Komatsu, K. J.;Krishnan, S.;Krishnan, S.;Laihonen, M.;Lecomte, N.;Lecomte, N.;Lehn, C. R.;Li, X.;Lindroth, R. L.;LoPresti, E. F.;Losada, M.;Sobral, M.;Louthan, A. M.;Lynch, S. C.;Lynn, J. S.;Vandvik, V.;Lynn, J. S.;Martin, B. S.;Pan, V. S.;Zarnetske, P. L.;Wetzel, W. C.;Massad, T. J.;McCall, A. C.;Merwin, A. C.;Moore, C. M.;Moreira, X.;Moshobane, M. C.;Moshobane, M. C.;Muola, A.;Nakadai, R.;Nakajima, K.;Nakajima, K.;Novais, S.;Ogbebor, C. O.;Ohsaki, H.;Yamawo, A.;Pan, V. S.;Zarnetske, P. L.;Wetzel, W. C.;Pardikes, N. A.;Pareja, M.;Parthasarathy, N.;Paynter, Q.;Pearse, I. S.;Pepi, A. A.;Phartyal, S. S.;Piper, F. I.;Piper, F. I.;Piper, F. I.;Puy, J.;Quintero, C.;Sasal, Y.;Rosche, C.;Runyon, J. B.;Sadeh, A.;Segre, H.;Sakata, Y.;Salgado-Luarte, C.;Sapir, Y.;Sato, Y.;Sawant, M.;Schumann, I.;Segoli, M.;Shinohara, N.;Singh, R. P.;Stotz, G. C.;Tack, A. J. M.;van Dijk, L. J. A.;Tayal, M.;Torrico-Bazoberry, D.;Tougeron, K.;Tougeron, K.;Trowbridge, A. M.;Uyi, O.;Vaca-Uribe, J. L.;van Dijk, L. J. A.;Villellas, J.;Waller, L. P.;Weber, M. G.;Yamawo, A.;Zhong, Z.;Zhong, Z.;Wetzel, W. C.;Wetzel, W. C.
关键词:
-
Effects of coated sodium butyrate on growth performance, intestinal morphology, and caecal short-chain fatty acids of growing Pekin ducks
作者:Ji, Z. Q.;Hao, Y. S.;Wang, Y. T.;Zhang, B.;Tang, J.;Huang, W.;Zhang, Q.;Hou, S. S.;Xie, M.;Wang, Y. T.;Xie, M.
关键词:Ducks; coated sodium butyrate; growth performance; intestinal morphology; short-chain fatty acids
-
Apparent metabolizable energy requirement of feed-restricted White Pekin duck breeder pullets
作者:Xie, M.;Tang, J.;Guo, Z. B.;Huang, W.;Zhang, Q.;Hou, S. S.;Meng, R. Z.;Xie, M.;Tang, J.;Guo, Z. B.;Huang, W.;Zhang, Q.;Hou, S. S.
关键词:Duck breeder pullets; Energy; Requirement
-
Function of yeast extract in greater wax moth farming: does yeast extract always improve the development and reproduction of insects?
作者:Xue, Q. Q.;Song, F.;Xue, Q. Q.;Men, L. N.;Zhang, Q.;Qi, J. Y.;Zhang, Z. W.;Guncan, A.;Zhang, Q.;Zhang, Y. H.;Deng, A.;Zhang, Z. W.
关键词:Galleria mellonella L.; yeast extract; age-stage two-sex life table; insect farming