Ectopic expression of sorbitol-6-phosphate 2-dehydrogenase gene from Haloarcula marismortui enhances salt tolerance in transgenic Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Gao, Jian-jie

作者： Gao, Jian-jie;Sun, Yu-ru;Zhu, Bo;Peng, Ri-He;Wang, Bo;Wang, Li-Juan;Li, Zhen-Jun;Yao, Quan-Hong;Sun, Yu-ru;Chen, Lei;Gao, Jian-jie;Zhu, Bo;Peng, Ri-He;Wang, Bo;Wang, Li-Juan;Li, Zhen-Jun;Yao, Quan-Hong

作者机构：

关键词： Sorbitol-6-phosphate 2-dehydrogenase; Transgenic Arabidopsis; Salt stress; Haloarcula marismortui

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN： 0137-5881

年卷期： 2018 年 40 卷 6 期

页码：

收录情况： SCI

摘要： D-Sorbitol-6-phosphate 2-dehydrogenase (S6PDH, E.C. 1.1.1.140) catalyzes the NADH-dependent conversion of D-fructose 6-phosphate (F6P) to D-sorbitol 6-phosphate (S6P). In this work, recombination and characterization of Haloarcula marismortui D-sorbitol-6-phosphate 2-dehydrogenase are reported. Haloarcula marismortui D-sorbitol-6-phosphate 2-dehydrogenase was expressed in P. pastoris and Arabidopsis thaliana. Enzyme assay indicated that HmS6PDH catalyzes the reduction of D-fructose 6-phosphate to D-sorbitol 6-phosphate and HmS6PDH activity was enhanced by NaCl. Furthermore, transgenic A. thaliana ectopic expressing HmS6PDH accumulate more sorbitol under salt stress. These results suggest that the ectopic expression of HmS6PDH in plants can facilitate future studies regarding the engineering and breeding of salt-tolerant crops.

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