Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes)

文献类型： 外文期刊

第一作者： Yang, Hairui

作者： Yang, Hairui;Zhang, Jinsong;Zhang, Henan;Yang, Yan;Wu, Di;Liu, Yanfang;Wang, Wenhan;Jia, Wei;Yang, Hairui;Zhang, Jinsong;Zhang, Henan;Yang, Yan;Wu, Di;Liu, Yanfang;Wang, Wenhan;Jia, Wei;Yang, Hairui;Zhang, Yaping;Sun, Wenbo;Zheng Qiankun;Min, Li

作者机构：

关键词： apoptosis; autophagy; By-1; cell proliferation; medicinal mushrooms; Taiwanofungus camphoratus

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）

ISSN： 1521-9437

年卷期： 2018 年 20 卷 4 期

页码：

收录情况： SCI

摘要： Taiwanofungus camphoratus has been reported to have antitumor effects against various cancer cells. The aim of this study was to investigate the direct inhibitory effect of By-1 (3-isobutyl-l-methoxy-4-[4'-(3-methylbut-2-enyloxy)phenyl]-1 H-pyrrole-2,5-dione), a compound from spent broth from submerged cultures of T. camphoratus, on human lung adenocarcinoma cells and to determine the molecular mechanism underlying this effect. The growth-inhibitory assay and colony formation assay showed that cell viability was significantly decreased. A By-1 concentration of 300 mu mol/L caused 73.55% cell death and at a concentration of 240 mu mol/L led to a 58% reduction in the number of colonies. The wound-healing assay showed that the distance of migration was 0.3 times shorter than that of untreated cells. Flow cytometry revealed that By-1 could suppress DNA synthesis, cause cell cycle arrest at the S phase, and induce apoptosis in a reactive oxygen sped es-dependent manner. Furthermore, the expression of caspase-3 and P53 was 4 times higher than that in untreated cells, and the antiapoptotic protein Bcl-2 was decreased 2 times compared with the protein in untreated cells. It is interesting to note that apoptosis and autophagy were both induced during treatment with By-1, and autophagy inhibition decreased cell proliferation. By-1 potently inhibited the growth of SPCA-1 cells by inducing cell cycle arrest and apoptosis. The combination of proapoptosis agents and antiautophagy agents could effectively enhance anticancer efficacy, which may be a new strategy in treating non-small cell lung cancer.

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