Cloning, characterization and functional analysis of dctn5 in immune response of Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Wei, Min

作者： Wei, Min;Xu, Wen-teng;Li, Kun-ming;Chen, Ya-dong;Wang, Lei;Meng, Liang;Zhao, Fa-zhen;Chen, Song-lin;Xu, Wen-teng;Li, Kun-ming;Chen, Ya-dong;Wang, Lei;Meng, Liang;Zhao, Fa-zhen;Chen, Song-lin;Wei, Min;Wei, Min;Li, Kun-ming

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关键词： Dynactin subunit 5; Chinese tongue sole; Expression pattern; Immunity; Antimicrobial activity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 77 卷

页码：

收录情况： SCI

摘要： In mammals, microtubule-dependent trafficking could participate the immune response, where the motor proteins are suggested to play an important role in this process, while the related study in fish was rare. In this study, dctn5, a subunit of dyactin complex for docking motor protein, was obtained by previous immune QTL screening. The full-length cDNAs of two dctn5 transcript variants were cloned and identified (named dctn5_tv1 and dctn5_tv2, respectively). Tissue distribution showed that dctn5_tv1 was widely distributed and high transcription was observed in immune tissue (skin), while dctn5_tv2 was predominantly detected in gonad and very low in other tissues. Time-course expression analysis revealed that dctn5_tv1 could be up-regulated in gill, intestine, skin, spleen, and kidney after Vibrio harveyi challenge. Moreover, recombinant Dctn5tv1 exhibited high antimicrobial activity against Escherichia coli and Streptococcus agalactiae due to binding to bacteria cells. Taken together, these data suggest Dctn5_tv1 is involved in immune response of bacterial invasion in Chinese tongue sole.

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