Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

文献类型： 外文期刊

第一作者： Sui, Xiukun

作者： Sui, Xiukun;Xin, Ting;Guo, Xiaoyu;Jia, Hong;Li, Ming;Gao, Xintao;Wu, Jing;Jiang, Yitong;Willems, L.;Zhu, Hongfei;Hou, Shaohua;Sui, Xiukun;Wu, Jing;Willems, L.

作者机构：

关键词： genomics; phylogeny; porcine respiratory and reproductive syndrome virus; virulence

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

ISSN： 1229-845X

年卷期： 2018 年 19 卷 3 期

页码：

收录情况： SCI

摘要： Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N-33 -> K-33), and a K151 amino acid (AA) substitution for virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the Nsp2-coding region. Inoculation experiments were conducted and weaker virulence of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV strain. The Nsp2 and GP5 differences, in addition to the virulence difference between these two highly pathogenic PRRSV strains, have the potential to be used to establish a basis for further study of PRRSV virulence determinants and to provide data useful in the development of vaccines against this economically devastating disease.

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