ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

文献类型： 外文期刊

第一作者： Jiang, Tian

作者： Jiang, Tian;Zhang, Jing;Rong, Liwei;Zhang, Lixin;Ouyang, Min;Jiang, Tian;Zhang, Jing;Rong, Liwei;Zhang, Lixin;Feng, Yanjiang;Wang, Qi;Song, Qiulai

作者机构：

关键词： Arabidopsis; chloroplast development; cotyledon; Early Chloroplast Development 1 (ECD1); early stages; pentatricopeptide repeat protein (PPR); RNA editing

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2018 年 69 卷 12 期

页码：

收录情况： SCI

摘要： Chloroplast development is a highly complex process and the regulatory mechanisms have not yet been fully characterized. In this study, we identified Early Chloroplast Development 1 (ECD1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 in Arabidopsis led to embryo lethality, and abnormal embryogenesis occurred in ecdl/+ heterozygous plants. A decrease in ECD1 expression induced by RNAi resulted in seedlings with albino cotyledons but normal true leaves. The aberrant morphology and under-developed thylakoid membrane system in cotyledons of RNAi seedlings suggests a role of ECD1 specifically in chloroplast development in seedlings. In cotyledons of ECD1-RNAi plants, RNA-editing of rps14-149 (encoding ribosomal protein S14) was seriously impaired. In addition, dramatically decreased plastid-encoded RNA polymerase-dependent gene expression and abnormal chloroplast rRNA processing were also observed. Taken together, our results indicate that ECD1 is indispensable for chloroplast development at the seedling stage in Arabidopsis.

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