Genotypic analysis of degenerative Cordyceps militaris cultured in the pupa of Bombyx mori

文献类型： 外文期刊

第一作者： Yin, Juan

作者： Yin, Juan;Xin, Xiang-Dong;Weng, Yu-Jie;Li, Shao-Hui;Jia, Jun-Qiang;Gui, Zhong-Zheng;Jia, Jun-Qiang;Gui, Zhong-Zheng

作者机构：

关键词： Analysis; Cordyceps militaris; Degenerative; Genotypic

期刊名称：ENTOMOLOGICAL RESEARCH （ 影响因子：1.306； 五年影响因子：1.242 ）

ISSN： 1738-2297

年卷期： 2018 年 48 卷 3 期

页码：

收录情况： SCI

摘要： The chemical composition and pharmacological effects of Cordyceps militaris are similar to those of Cordyceps sinensis, with the former undergoing greater development and utilization. Strain degeneration is a common phenomenon that occurs with high frequency during the subculturing of C. militaris, however, and the mechanism underlying strain degeneration remains unclear. In this study, we used touch-down PCR to compare the ITS1+5.8S+ITS2, 18S, 28S and mating-type (MAT) regions sequence of wild-type and degenerated strains of C. militaris. We also used quantitative real-time PCR to analyze expression levels of the CmMAT gene. Sequence analysis showed that the ITS1+5.8S+ITS2 and 28S regions of degenerated and wild-type strains were completely identical, the 18S region of the degenerated strain contained seven single-base mutations, including six base substitutions and one single-base insertion. Compared with the wild-type strain, the degenerated strain contained a deletion of the MAT1-2-1 region, three base substitutions in the MAT1-1-1 region, and a base substitution in the MAT1-1-2 region that causes a glycine-to-valine amino acid substitution. Quantitative real-time PCR analysis detected no CmMAT1-2-1 gene expression in the degenerated strain, confirming the deletion of the CmMAT1-2-1 gene. Expression levels of the CmMAT1-1-1 and CmMAT1-1-2 genes were significantly down-regulated to only 7.5% and 4.4%, respectively, that of the wild-type strain. These results indicate that 18S and MAT region mutations, as well as down-regulated of CmMAT gene expression levels, may play important roles in C. militaris degeneration. This study provides a theoretical basis for further elucidation of the molecular mechanisms of C. militaris degeneration.

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