Development of glyphosate-resistant alfalfa (Medicago sativa L.) upon transformation with the GR79Ms gene encoding 5-enolpyruvylshikimate-3-phosphate synthase

文献类型： 外文期刊

第一作者： Yi, Dengxia

作者： Yi, Dengxia;Ma, Lin;Li, Cong;Lin, Min

作者机构：

关键词： GR79Ms; Agrobacterium tumefaciens-mediated transformation; Transgenic alfalfa; Glyphosate tolerance; Molecular analysis

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN： 0032-0935

年卷期： 2018 年 248 卷 1 期

页码：

收录情况： SCI

摘要： The glyphosate-resistant gene, GR79Ms, was successfully introduced into the genome of alfalfa. The transgenic events may serve as novel germplasm resources in alfalfa breeding. Weed competition can reduce the alfalfa yield, generating new alfalfa germplasm with herbicide resistance is essential. To obtain transgenic alfalfa lines with glyphosate resistance, a new synthetic glyphosate-resistant gene GR79Ms encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into alfalfa germplasm by Agrobacterium tumefaciens-mediated transformation. In total, 67 transformants were obtained. PCR and Southern blot analyses confirmed that GR79Ms was successfully inserted into the genome of alfalfa. Reverse transcription-PCR and western blot analyses further demonstrated the expression of GR79Ms and its product, GR79Ms EPSPS. Moreover, two homozygous transgenic lines were developed in the T-2 generation by means of molecular-assisted selection. Herbicide tolerance spray tests showed that the transgenic plants T-0-GR1, T-0-GR2, T-0-GR3 and two homozygous lines were able to tolerate fourfold higher commercial usage of glyphosate than non-transgenic plants.

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