Nanobody Technology for Mycotoxin Detection: Current Status and Prospects

文献类型： 外文期刊

第一作者： He, Ting

作者： He, Ting;Zhu, Jiang;Nie, Yao;Hu, Rui;Yang, Yunhuang;Wang, Ting;Li, Peiwu;Zhang, Qi

作者机构：

关键词： Nanobody; Mycotoxins; Immunoassay; Food safety

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2018 年 10 卷 5 期

页码：

收录情况： SCI

摘要： Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. However, conventional antibodies involved in immunoassays are difficult to be expressed recombinantly and are susceptible to harsh environments. Nanobodies (or VHH antibodies) are antigen-binding sites of the heavy-chain antibodies produced from Camelidae. They are found to be expressed easily in prokaryotic or eukaryotic expression systems, more robust in extreme conditions, and facile to be used as surrogates for artificial antigens. These properties make them the promising and environmentally friendly immunoreagents in the next generation of immunoassays. This review briefly describes the latest developments in the area of nanobodies used in mycotoxin detection. Moreover, by integrating the introduction of the principle of nanobodies production and the critical assessment of their performance, this paper also proposes the prospect of nanobodies in the field of food safety in the foreseeable future.

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