文献类型： 外文期刊
第一作者： Wan, Chunhe
作者： Wan, Chunhe;Cheng, Longfei;Fu, Guanghua;Chen, Cuiteng;Liu, Rongchang;Shi, Shaohua;Chen, Hongmei;Fu, Qiuling;Huang, Yu
作者机构：
关键词： Goose hemorrhagic polyomavirus; TaqMan real-time PCR; Sensitivity; Domestic Jinyun Sheldrakes
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2018 年 39 卷
页码：
收录情况： SCI
摘要： Due to low doses of infection, an efficient and sensitive virus detection method is necessary to detect low amounts of goose hemorrhagic polyomavirus (GHPV). In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses. The standard curve had a linear correlation of 0.997 and efficiency of 99% between the cycle threshold value and the logarithm of the plasmids copy number. The possible lowest detectable concentration was 35.4 copies/mu l; 100 times more sensitive than conventional PCR (detection limit, 3.54 x 10(3) copies/mu l). Domestic Jinyun Sheldrakes ducks and their embryonated eggs were found positive of GHPV infection which provides evidence of possible vertical transmission of GHPV.
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