Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity

文献类型： 外文期刊

第一作者： Nan, Liangliang

作者： Nan, Liangliang;Chen, Chen;Li, Yafei;Zhou, Enmin;Zhang, Gaiping;Nan, Liangliang;Liu, Yunchao;Feng, Hua;Cui, Yinglei;Wang, Yanwei;Li, Yafei;Zhang, Gaiping;Ji, Pengchao;Cui, Yinglei;Zhang, Gaiping;Wang, Juan;Liu, Dongmin

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关键词： Canine parvovirus; Escherichia coli; Immunogenicity; Trigger factor 16; Virus-like particle

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2018 年 15 卷

页码：

收录情况： SCI

摘要： Canine parvovirus (CPV) has been considered to be an important pathogen, which can cause acute infectious disease in canids. Although current vaccines are effective in preventing CPV infection, safety problems still remain unsolved. In this study, a subunit vaccine against CPV based on virus-like particles (VLPs) with good safety and immunogenicity is reported. Soluble CPV VP2 protein was produced by co-expression of chaperone trigger factor (Tf16) in Escherichia coli (E.coli), and assembled into CPV VLPs which could be affected by NaCl and pH. At 250 mM NaCl pH 8.0, the VLPs co-expressed with Tf16 had similar size (25 nm) and shape with the authentic virus capsid under the transmission electron microscopy (TEM), which is also in accordance with the dynamic light scattering (DLS) data. Immunization with these particles could induce high-titer hemagglutination inhibition (1:12288) and neutralizing antibodies (1:6144) in guinea pigs. Splenic cells of them could secrete IFN-gamma and IL-4 after stimulation by CPV. Thus, the VLPs produced by the new approach with high yield and immunogenicity could be a potential candidate for CPV vaccine.

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