Identification and Characterization of Drought Stress-Responsive Novel microRNAs in Dongxiang Wild Rice

文献类型： 外文期刊

第一作者： Zhang Fantao

作者： Zhang Fantao;Luo Yuan;Zhang Meng;Zhou Yi;Xie Jiankun;Chen Hongping;Hu Biaolin

作者机构：

关键词： Dongxiang wild rice; drought stress; genetic resource; novel microRNA; small RNA sequencing

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2018 年 25 卷 4 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are non-coding small RNAs, which play important regulatory roles in response to biotic and abiotic stresses. Dongxiang wild rice (Oryza rufipogon, DXWR) can survive in extreme drought environment, but its molecular mechanism of drought resistance is still largely unknown. To further explore miRNA regulatory mechanisms involved in drought resistance, we identified 138 novel miRNAs in DXWR using small RNA sequencing and bioinformatics approaches, and found that the expression levels of 67 novel miRNAs were signicantly affected by drought stress. In total, 200 candidate target genes were predicted and annotated for the drought stress-responsive novel miRNAs. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways suggested that most of the target genes were related to metabolism. Stem-loop quantitative real-time PCR (qRT-PCR) results exhibited high concordance with sequencing data, which conrmed that miRNA expression patterns based on small RNA sequencing in the present study were reliable. Meanwhile, qRT-PCR validated the inverse expression patterns between several miRNAs and their target genes. These results will enhance our understanding of miRNA regulatory mechanisms in response to drought stress in DXWR, and can serve as an important reference for the protection and utilization of this valuable genetic resource.

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