Detection of plant protein adulterated in fluid milk using two-dimensional gel electrophoresis combined with mass spectrometry

文献类型： 外文期刊

第一作者： Yang, Jinhui

作者： Yang, Jinhui;Zheng, Nan;Wang, Jiaqi;Yang, Jinhui;Zheng, Nan;Wang, Jiaqi;Yang, Jinhui;Zheng, Nan;Wang, Jiaqi;Yang, Jinhui;Soyeurt, Helene;Yang, Yongxin

作者机构：

关键词： Milk adulteration; Plant protein; Two-dimensional gel electrophoresis; Mass spectrometry

期刊名称：JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE （ 影响因子：2.701； 五年影响因子：3.574 ）

ISSN： 0022-1155

年卷期： 2018 年 55 卷 7 期

页码：

收录情况： SCI

摘要： The illegal or unlabelled addition of plant protein in milk can cause serious anaphylaxis. For sustainable food security, it is therefore important to develop a methodology to detect non-milk protein in milk products. This research aims to differentiate milk adulterated with plant protein using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. According to the protein spots highlighted on the gel of adulterated milk, beta-conglycinin and glycinin were detected in milk adulterated with soy protein, while legumin, vicilin, and convicilin indicated the addition of pea protein, and beta-amylase and serpin marked wheat protein. These results suggest that a 2-DE-based protein profile is a useful method to identify milk adulterated with soy and pea protein, with a detection limit of 4% plant protein in the total protein.

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