A cationic conjugated polymer and graphene oxide: Application to amplified fluorescence detection of sinapine

文献类型： 外文期刊

第一作者： Zhang, Zhen

作者： Zhang, Zhen;Xiang, Xia;Huang, Fenghong;Xia, Xiaoyang;Zheng, Mingming;Han, Ling;Tang, Hu;Shi, Jianbin

作者机构：

关键词： SP; GO; PFP; FRET; Fluorescence amplification

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2018 年 203 卷

页码：

收录情况： SCI

摘要： An amplified fluorescence strategy is described for the detection of sinapine (SP) by using a cationic conjugated polymer (PFP) and graphene oxide (GO). It is observed that the fluorescein (FAM)-labeled single-stranded DNA (FAM-DNA) is absorbed on the surface of GO if SP is absent. This causes that fluorescence resonance energy transfer (FRET) from PFP to FAM is inefficient when adding PFP into FAM-DNA/GO complex. If SP is added to FAM-DNA/GO complex, FAM-DNA is desorbed from GO surface due to the competitive binding of SP and FAM-DNA toward GO. In this case, FAM-DNA is close to PFP in the presence of PFP through strong electrostatic interaction, leading to the occurrence of efficient FRET. Based on the above phenomenon, we demonstrate a method to amplify fluorescence signal of traditional GO-based SP assay by introducing PFP. In comparison to the use of single GO, the combination of PFP with GO-based strategy displays high turn-on ratio and enhanced sensitivity with a limit of detection as low as 73 ng mL(-1) for SP detection. Satisfactory results in practical samples are also obtained by the recovery experiments, demonstrating the potential application of cationic conjugated polymer in plant-derived small molecule. (C) 2018 Elsevier B.V. All rights reserved.

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