Comparative transcriptome analysis reveals significant differences in gene expression between appressoria and hyphae in Colletotrichum gloeosporioides

文献类型： 外文期刊

第一作者： Ji, Zhirui

作者： Ji, Zhirui;Zhang, Junxiang;Wang, Meiyu;Zhou, Zongshan;Wu, Jianyuan;Zhang, Junxiang

作者机构：

关键词： Glomerella; ABC transporter; Effector; Transcription factor; Anthracnose

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 670 卷

页码：

收录情况： SCI

摘要： Fruit rot caused mainly by Colletotrichum gloeosporioides is a major cause of pre- and/or post-harvest diseases, which seriously constrains production, marketing, and export of fruits. To infect the host, this fungus evolves a specialized infection structure called the appressorium. Extensive past studies have characterized many appressorium-related Fruit rot caused mainly by Colletotrichum gloeosporioides is a major cause of pre- and/or post-harvest diseases, which seriously constrains production, marketing, and export of fruits. To infect the host, this fungus evolves a specialized infection structure called the appressorium. Extensive past studies have characterized many appressorium-related genes in C. gloeosporioides, separately. However, a comprehensive understanding of the genes contributing to appressorium formation is far from complete. Here, global changes in gene expression were analyzed between appressoria and hyphae using RNA-Seq. We identified 4071 genes that are up-regulated in appressorium and discovered 468 unigenes that are expressed only in appressoria, compared with the fungal hyphae. Differentially expressed genes between appressoria and hyphae were assigned to 107 KEGG pathways, including metabolic pathways, secondary metabolite biosynthesis, molecular transport and signal transduction. Fourteen putative ABC transporter genes are significantly up-regulated in appressoria, and in contrast, twenty six down-regulated. One hundred and one transcription factor genes show more than a 2-fold up-regulation in appressoria compared to hyphae. The up-regulation of 39 secreted protein candidates is observed, suggesting they may play important roles in initial infection processes. Our data demonstrate that appressorium development of C. gloeosporioides is accompanied by significant changes in gene expression, which provides novel insights to elucidate how this fungus regulates its development, pathogenicity and immune evasion.

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