Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic Toxoplasma gondii infection in Kunming mice

文献类型： 外文期刊

第一作者： Li, Zhong-Yuan

作者： Li, Zhong-Yuan;Li, Zhong-Yuan;Lu, Jing;Zhang, Nian-Zhang;Hou, Jun-Ling;Guo, Hai-Ting;Zhu, Xing-Quan;Elsheikha, Hany M.;Guo, Hai-Ting

作者机构：

关键词： Toxoplasma gondii; HSP40; DNA vaccine; Chronic infection; Immune evaluation

期刊名称：PARASITE （ 影响因子：3.0； 五年影响因子：3.046 ）

ISSN： 1252-607X

年卷期： 2018 年 25 卷

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收录情况： SCI

摘要： Toxoplasma gondii causes one of the most common protozoal diseases of humans and animals worldwide. With the aim of designing an effective vaccine against T. gondii infection, we examined the immunogeincity of a DNA vaccine expressing heat shock protein 40 (HSP40) against challenge with T. gondii (type I RH and type II Pru) strains in Kunming mice. The plasmid pVAXl-HSP40 was constructed and used to immunize mice by intramuscular injection for three sequential immunizations with two-week intervals. This immunization regimen significantly reduced parasite cyst burden in pVAXl-HSP40-immumzed mice (1871.9 +/- 142.3) compared with control mouse groups immunized with pVAXl (3479.2 +/- 204.4), phosphate buffered saline (3024.4 +/- 212.8), or left untreated (3275.0 +/- 179.8) as healthy controls (p < 0.01). However, immunization failed to protect mice against challenge with the virulent RH strain. There was a significant increase in T lymphocyte subclasses (CD3e(+)CD4(+) T and CD3e(+)CD8a(+) T lymphocytes) in splenic tissues in immunized mice compared with controls (p < 0.05). However, the level of antibodies, lymphocyte proliferation and concentration of cytokines (IFN-gamma, IL-2, IL-4, IL-10 and IL-12p70) were not significantly different between immunized and control mouse groups (p < 0.05). These data indicate that pVAXl-HSP40 induced specific immune responses and achieved a significant reduction m the number of brain cysts in Pru-infected mice, and thus can be tested in future immunization studies along with plasmids containing other immunogenic proteins as a cocktail vaccine to fully abolish chronic toxoplasmosis.

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