Waxy allelic diversity in common millet (Panicum miliaceum L.) in China

文献类型： 外文期刊

第一作者： Wang, Ruiyun

作者： Wang, Ruiyun;Liu, Xiaohuan;Ji, Xu;Wang, Ruiyun;Wang, Haigang;Chen, Ling;Qiao, Zhijun;Lu, Ping;Liu, Minxuan;Teng, Bin

作者机构：

关键词： Common millet; Waxy gene; SNP; Starch

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2018 年 6 卷 4 期

页码：

收录情况： SCI

摘要： A set of 132 accessions of common millet, Panicum miliaceum L., from 12 provinces of China were assessed for endosperm starch type (waxy or non-waxy) using I-2-HI staining, amylose and amylopectin contents using the dual-wavelength coloilmetric method, and genotype of Waxy genes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L (intron 5-exon 7 and intron 8-9) and Wx-S (exon 9-intron 10) revealed several polymorphisms (S-0, S-15, L-C, L-F, L-Y). Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S-15 allele had waxy endosperm starch with the amylose content range 0-2.58% and 64 accessions with the So allele had non-waxy endosperm starch with amylose content range 3.94%-23.29%. Five genotypes including S-15/L-F (45% of the accessions), S-0/L-F (25%), S-0/L-Y (12%), S-0/L-C (11%), and S-15/L-Y (7%) were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose. (C) 2018 "Crop Science Society of China and Institute of Crop Science, CAAS". Production and hosting by Elsevier B.V.

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