The transcriptome of flower development provides insight into floral scent formation in Freesia hybrida

文献类型： 外文期刊

第一作者： Huang, Minling

作者： Huang, Minling;Fan, Ronghui;Ye, Xiuxian;Lin, Rongyan;Luo, Yuanhua;Fang, Nengyan;Zhong, Huaiqin;Chen, Shilin

作者机构：

关键词： Freesia hybrida; Floral scent; Transcriptome sequencing; Monoterpenes; Secondary metabolism

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

ISSN： 0167-6903

年卷期： 2018 年 86 卷 1 期

页码：

收录情况： SCI

摘要： Freesia hybrida is a popular cut flower that is globally cultivated. Despite fragrance being an important floral trait, little is known of its associated molecular mechanisms in F. hybrida. In this study, cDNA libraries were constructed for three floral developmental stages in F. hybrida. A total of 74,660 unigenes were obtained from RNA sequencing and de novo assembly, of which 72.20% were annotated by seven public protein databases. Approximately 12,420 differentially expressed genes were identified during flower development. Gas chromatography-mass spectrometry analysis detected a total of 34 floral volatile compounds, primarily volatile monoterpenes, which accounted for approximately 90% of the volatiles. Using a system analysis-based approach, 36 candidate genes related to volatile terpenes were identified, in which the majority of up-regulated genes associated with the biosynthesis of monoterpenes and the majority of down-regulated genes associated with the biosynthesis of sesquiterpenes. Of these, FhDXS2A, FhGPPs and FhTPSs were considered to be important in floral scent formation. This study provides an in-depth assessment of floral scent and a reference for the future molecular breeding of floral scent in ornamental plants.

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