A Gustatory Receptor Used for Rapid Detection of Tyrophagus putrescentiae in Fungi Hosts

文献类型： 外文期刊

第一作者： Qu, Shao-Xuan

作者： Qu, Shao-Xuan;Li, Hui-Ping;Luo, Xin;Ma, Lin;Wang, Xiao-Fei

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： The storage mite, Tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in China. Storage mites are tiny and difficult to observe, especially when they occur in fungi composts. In this study, one gustatory receptor protein (TputGR1) was identified from the transcriptome of T. putrescentiae. Phylogenetic analysis of GRs families from 10 arthropod species revealed that TputGR1 had high homology with the SccaGR1 of Sarcoptes scabiei and TurtGR1-2 of Tetranychus urticae, but low homology with other insect species, Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Aedes aegypti, Culex quinquefasciatus, and Pediculus humanus. We developed a detection system for the mite on fungi hosts using the GR protein and the loop-mediated isothermal amplification (LAMP). This procedure was rapid (60 min from sampling to result) and had high sensitivity (0.5 ng/mL). LAMP provided rapid and reliable detection of T. putrescentiae. It has good specificity for single samples and for large-scale surveys.

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