Mercury ion-mediated "molecular beacon" integrating with hybridization chain reaction: Application to fluorescence turn-on detection of glutathione by using quantum dots and Ru complex

文献类型： 外文期刊

第一作者： Zhang, Zhen

作者： Zhang, Zhen;Xiang, Xia;Huang, Fenghong;Zheng, Mingming;Xia, Xiaoyang;Han, Ling

作者机构：

关键词： Mercury ion; MBs; HCR; Quantum dots; Cellular GSH levels; Fluorescence imaging

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN： 0925-4005

年卷期： 2018 年 273 卷

页码：

收录情况： SCI

摘要： A fluorescence turn-on strategy is described for the detection of glutathione (GSH) based on mercury ion (Hg2+)mediated molecular beacons (MBs) integrating with hybridization chain reaction (HCR) coupled with quantum dots (QDs) and Ru complex. Specifically, the thymine-Hg2+-thymine (T-Hg2+-T) ligation facilitates the single stranded oligonucleotide into MBs with a hairpin structure if GSH is absent. This prevents the occurrence of HCR on magnetic microparticles (MMPs), and thus allows the fluorescence quenching of Ru toward QDs in supernate. If, However, GSH is present, the competition binding between GSH and Hg2+ changes MBs strand into open form, resulting in the hybridization with another single-stranded DNA conjugated on MMPs with a sticky tail left. Hence, double-stranded (dsDNA) polymers on MMPs are formed through HCR. Obvious fluorescence increase of QDs is obtained due to the strong affinity of Ru complex to dsDNA on MMPs. The fluorescence intensity of QDs is directly related to GSH concentration. Under the optimal conditions, the approach exhibits high sensitivity to GSH with a detection limit of 8.2 nM. This method is further employed to evaluate cellular GSH levels by analyzing cell lysates, demonstrating good consistencies with fluorescence imaging and commercial GSH kit

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