Rat Small Intestinal Mucosal Epithelial Cells Absorb Dietary 1,3-Diacylglycerol Via Phosphatidic Acid Pathways

文献类型： 外文期刊

第一作者： Xu, Tongcheng

作者： Xu, Tongcheng;Li, Jianpeng;Qiu, Bin;Liu, Wei;Liu, Zhenhua;Du, Fangling;Li, Jianpeng;Zou, Jiayong;Lin, Xinying;Li, Duo;Liu, Zhenhua

作者机构：

关键词： Diacylglycerol; Diacylglycerol acyltransferase-2; Phosphatidic acid pathways; Small intestine

期刊名称：LIPIDS （ 影响因子：1.88； 五年影响因子：2.417 ）

ISSN： 0024-4201

年卷期： 2018 年 53 卷 3 期

页码：

收录情况： SCI

摘要： Compared with triacylglycerol (TAG), dietary 1,3-diacylglycerol (1,3-DAG) is associated with reduced serum lipid and glucose levels. We investigated the metabolism of 1,3-DAG by assaying its intermediate metabolites during digestion and absorption in the rat small intestine. After gavage with TAG emulsion, TAG was digested mainly to 2-monoacylglycerol (2-MAG) and unesterified fatty acid (FFA) in the rat small intestinal lumen. 2-MAG was directly absorbed into the small intestinal epithelial cells and esterified to 1,2(2,3)-DAG, and further esterified to TAG. After gavage with 1,3-DAG emulsion, 1,3-DAG was digested mainly to 1(3)-MAG and FFA in the rat small intestinal lumen with subsequent significant increase of 1-MAG and 1,3-DAG concentrations in small intestinal mucosal epithelial cells, and the 2-MAG, 1,2(2,3)-DAG, and TAG concentrations in mucosal epithelial cells were not significantly different after 1,3-DAG than after TAG gavage, suggesting that the metabolic pathway of 1,3-DAG is different from that of TAG. In intestinal mucosal epithelial cells, we further assayed enzyme levels and gene expression of proteins in the phosphatidic acid (PtdOH) pathway. The glycerol kinase, phosphatidate phosphatase, and diacylglycerol acyltransferase-2 expression and the relative expression of mRNA of enzymes were significantly increased in the 1,3-DAG group compared with the TAG group, suggesting that TAG synthesis from dietary 1,3-DAG was mainly via PtdOH pathways, which may partially account for the effect of dietary DAG on postprandial serum TAG.

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[1]Rat Small Intestinal Mucosal Epithelial Cells Absorb Dietary 1,3-Diacylglycerol Via Phosphatidic Acid Pathways. Xu, Tongcheng,Li, Jianpeng,Qiu, Bin,Liu, Wei,Liu, Zhenhua,Du, Fangling,Li, Jianpeng,Zou, Jiayong,Lin, Xinying,Li, Duo,Liu, Zhenhua. 2018

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