Development and validation of a UFLC-MS/MS method for simultaneous quantification of sixty-six saponins and their six aglycones: Application to comparative analysis of red ginseng and white ginseng

文献类型： 外文期刊

第一作者： Zhou, Qi-Le

作者： Zhou, Qi-Le;Yang, Xiu-Wei;Xu, Wei;Zhou, Qi-Le;Yang, Xiu-Wei;Xu, Wei;Zhou, Qi-Le;Zhu, Di-Na;Zhu, Di-Na;Wang, Ying-Ping

作者机构：

关键词： Red ginseng; UFLC-MS/MS; Ginsenoside markers; Quantification

期刊名称：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS （ 影响因子：3.935； 五年影响因子：3.563 ）

ISSN： 0731-7085

年卷期： 2018 年 159 卷

页码：

收录情况： SCI

摘要： A new and sensitive ultra fast liquid chromatography coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed to evaluate the quality of Red ginseng (RG) and to find out its chemical markers by comparing with multi-batches of RG and white ginseng (WG). This innovative method could quantify sixty-six saponins and their six aglycones including 10 pairs of 20(5) and 20(R) epimers within 35 min simultaneously. All compounds could be determined in individual multiple-reaction monitoring channel without interference, and the optimized method was rapid, accurate, precise, reproducible and efficient. Using the orthogonal partial least squared discriminant analysis, ginsenosides Rg(5), Rh-4, Rk(1), Rs(4), F-4, and 20(S)-Rg(3) were found to be the characteristic components of RG, the six compounds should be suggested as quality control markers to distinguish RG from WG. These findings will be significant for standardizing the processing procedures of RG and ensuring the consistent quality, as well as consequently the efficacy of RG in clinical applications. Results will be helpful in providing crucial chemical profiles of RG. (C) 2018 Elsevier B.V. All rights reserved.

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