MOLECULAR CHARACTERIZATION AND EXPRESSION ANALYSIS OF CATHEPSIN C IN THE MARINE CRAB CHARYBDIS JAPONICA (A. MILNE-EDWARDS, 1861) (DECAPODA, BRACHYURA, PORTUNIDAE)

文献类型： 外文期刊

第一作者： Chen, Jian H.

作者： Chen, Jian H.;Li, Ying L.;Chen, Jian H.;Chen, Hai G.;Chen, Jian H.;Gao, Huan;Li, Ying L.;Xu, Xing H.;Chen, Jian H.;Gao, Huan;Xu, Xing H.;Chen, Jian H.;Gao, Huan;Xu, Xing H.

作者机构：

关键词： Charybdis japonica; cathepsin C; Vibrio alginolyticus; Vibrio anguillarum; expression patterns

期刊名称：CRUSTACEANA （ 影响因子：0.529； 五年影响因子：0.65 ）

ISSN： 0011-216X

年卷期： 2018 年 91 卷 10 期

页码：

收录情况： SCI

摘要： Cathepsin C is a crucial lysosomal cysteine proteinase that takes part in protein degradation and proenzyme activation. In this study, cDNA of cathepsin C in Charybdis japonica (A. Milne-Edwards, 1861) (designated as Cj-cath C) was cloned and characterized. The results showed that the open reading frame (ORF) of Cj-cath C was 1356 bp and encoded a protein of 451 amino acids. Cj-cath C contained two catalytic residues, a Cathepsin C exclusion domain and a Peptidase_C1 domain. The Cj-cath C was widely expressed in all tissues, with highest expression in hepatopancreas and heart. The transcript levels of Cj-cath C in the hepatopancreas and haemolymph were up-regulated after stimulation by Vibrio anguillarum Bergeman, 1909 and reached a peak value at 6 h post-infection, followed by a gradual decrease. These observations indicate that Cj-cath C might be a constitutive and inducible acute-phase protein that is involved in the immune defense of C. japonica.

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