Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove

文献类型： 外文期刊

第一作者： Ma, Caiyun

作者： Ma, Caiyun;Guo, Yu;Tan, Leiqi;Wang, Chunjing;Liu, Changqing;Ma, Caiyun;Wen, Hebao;Zheng, Yanjie;Li, Xiangchen;Guan, Weijun;Guo, Yu;Tan, Leiqi;Wang, Chunjing;Liu, Changqing

作者机构：

关键词： goose inguinal groove; ADSCs; multilineage potential; regenerative therapies

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

ISSN： 1044-5498

年卷期： 2018 年 37 卷 9 期

页码：

收录情况： SCI

摘要： Adipose tissue-derived mesenchymal stem cells (ADSCs) play a crucial role in the field of regenerative medicine and tissue repair for its own unique features. However, up to date, the isolation and characterizations of multidifferentiation potentials of goose ADSCs are still uncertain. In this study, we successfully isolated ADSCs from goose inguinal groove in vitro for the first time and also attempted to unravel its fundamental differentiation potentials and genetic characteristics. The results showed that isolated ADSCs exhibited a typical fibroblast-like morphology and high proliferative potential, could be passaged for at least 40 passages and maintained high hereditary stability with more than 92.2% of cells were diploid (2n=78) by G-banding analysis. Moreover, the ADSCs could express pluripotent marker gene (OCT4) and mesenchymal stem cells-related surface antigens, which are similar to previously reported human ADSCs. Additionally, the goose ADSCs could be induced to transdifferentiate into cells of three layers in vitro, such as osteoblasts, chondrocytes, and adipocytes derived from mesoderm, neurocytes from ectoderm, and hepatocytes of the endoderm. Most of all, we confirmed that the induced -like cells and hepatocytes had metabolic functions similar to normal cells in vivo. Taken together, these results demonstrated the multidifferentiation potentials of ADSCs in vitro, which conferred an appealing candidate for cell regenerative therapy.

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