Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

文献类型： 外文期刊

第一作者： Liu, Juntao

作者： Liu, Juntao;Zhu, Lihui;Wang, Lihui;Chen, Yongjun;Gin, Bikash Ranjan;Cheng, Guofeng;Wang, Lihui;Li, Jianjun

作者机构：

关键词： Immunology and Infection; Issue 135; Extracellular vesicles; Isolation; Schistosomes; Characterization; In vitro culture; Diagnosis

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.355； 五年影响因子：1.696 ）

ISSN： 1940-087X

年卷期： 2018 年 135 期

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收录情况： SCI

摘要： Extracellular vesicles (EVs) are membranous vesicles released by a variety of cells into the extracellular microenvironment. EVs represent a population of heterogeneous vesicles, whose size range between 40 and 1,000 nm. Accumulated evidence indicated that EVs play important regulatory roles in pathogen-host interactions. A deep understanding of schistosome EVs should provide insights into the mechanisms underlying schistosome-host interactions, enabling development of novel strategies against schistosomiasis. Here, we aim to further study EVs functions in schistosomes by presenting a protocol for the isolation and characterization of EVs from adult Schistosoma japonicum (S. japonicum). EVs were isolated from in vitro culture medium using centrifugation combined with a commercial exosome isolation kit. The isolated S. japonicum EVs (SjEVs) typically possess a diameter of 100 - 400 nm, and are characterized by transmission electronic microscopy and western blotting. The usage of PKH67 dye-labeled SjEVs has demonstrated that SjEVs are internalized by the recipient cells. Overall, our protocol provides an alternative method for isolating EVs from adult schistosomes; the isolated SjEVs may be suitable for functional analysis.

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