Identification of SNPs involved in regulating a novel alternative transcript of P450 CYP6ER1 in the brown planthopper

文献类型： 外文期刊

第一作者： Pang, Rui

作者： Pang, Rui;Dong, Yi;Sun, Zhong-Xiang;Zhang, Wen-Qing;Ling, Yan

作者机构：

关键词： alternative splicing; cytochrome P450; Nilaparvata lugens; single nucleotide polymorphisms (SNPs); transcription factor binding

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2018 年 25 卷 5 期

页码：

收录情况： SCI

摘要： Cytochrome P450-mediated metabolic resistance is one of the major mechanisms involved in insecticide resistance. Although the up-regulation of cytochrome P450 plays a vital role in insecticide metabolism, the molecular basis for the transcriptional regulation of cytochrome P450 remains largely unknown. The P450 gene CYP6ER1, has been reported to confer imidacloprid resistance to the brown planthopper, Nilaparvata lugens. Here, we identified a novel alternative transcript of CYP6ER1 (transcript A2) that had different expression patterns between resistant and susceptible populations, and was more stable after insecticide induction. The promoter of this transcript was sequenced and multiple single nucleotide polymorphisms (SNPs) were detected in individuals from susceptible and resistant field-collected populations. Resistant alleles of four SNPs were found to significantly enhance the promoter activity of the CYP6ER1 transcript A2. Electrophoretic mobility shift assays (EMSAs) revealed that these SNPs might regulate the binding of transcription factors to the promoter. Our findings provide novel evidence regarding the transcriptional regulation of a metabolic resistance-related gene and may be useful to understand the resistance mechanism of N. lugens in the field.

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