Construction of a highly efficient display system for baculovirus and its application on multigene co-display

文献类型： 外文期刊

第一作者： Zheng, Hao

作者： Zheng, Hao;Wang, Xiong;Ren, Feifei;Feng, Min;Xu, Liangliang;Sun, Jingchen;Zheng, Hao;Wang, Xiong;Ren, Feifei;Feng, Min;Xu, Liangliang;Sun, Jingchen;Zou, Shenglong;Yao, Lunguang;Yao, Lunguang

作者机构：

关键词： Insect virus; Baculovirus multigene display system; Gene expression; High efficiency

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN： 1617-4615

年卷期： 2018 年 293 卷 5 期

页码：

收录情况： SCI

摘要： The classical baculovirus display system (BDS) has often recruited fields including gene delivery, gene therapy, and the genetic engineering of vaccines, as it is capable of presenting foreign polypeptides on the membranes of recombinant baculovirus through a transmembrane protein. However, classical BDS's high cost, complicated operation, low display efficiency and its inability to simultaneously display multiple gene products impede its practicality. In this study, we present a novel and highly efficient display system based on ires-dependent gp64 for rescuing gp64-null Bacmid of baculovirus construction without affecting the viral replication cycle, which we name the baculovirus multigene display system (BMDS). Laser scanning confocal microscopy demonstrated that eGFP, eYFP, and mCherry were translocated on the membrane of Spodoptera frugiperda 9 cell successfully as expected. Western blot analysis further confirmed the presence of the fluorescent proteins on the budded, mature viral particles. The results showed the display efficiency of target gene on cell surface is fourfold that of classical BDS. In addition, a recombinant baculovirus displaying three kinds of fluorescent proteins simultaneously was constructed, thereby demonstrating the effectiveness of BMDS as a co-display system.

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