Production of gamma-terpinene by metabolically engineered Escherichia coli using glycerol as feedstock

文献类型： 外文期刊

第一作者： Qi, Chang

作者： Qi, Chang;Zhao, Hongwei;Li, Xing;Zhang, Ge;Xian, Mo;Zhang, Haibo;Zhang, Ge;Liu, Haobao;Wang, Qian;Liu, Haobao;Xiang, Haiying;Wang, Yi;Li, Wenyang

作者机构：

期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

ISSN： 2046-2069

年卷期： 2018 年 8 卷 54 期

页码：

收录情况： SCI

摘要： Gamma ()-terpinene, a monoterpene compound, which is generally used in the pharmaceutical and cosmetics industries, due to its physical and chemical properties, is expected to become one of the more influential compounds used as an alternative biofuel in the future. It is necessary to seek more sustainable technologies such as microbial engineering for -terpinene production. In this study, we metabolically engineered Escherichia coli to produce -terpinene by introducing a heterologous mevalonate (MVA) pathway combined with the geranyl diphosphate synthase gene and -terpinene synthase gene. Subsequently, the culture medium and process conditions were optimised with a titre of 19.42 mg L-1 obtained. Additionally, in-depth analysis at translation level for the engineered strain and intermediate metabolites were detected for further analysis. Finally, the fed-batch fermentation of -terpinene was evaluated, where a maximum concentration of 275.41 mg L-1 with a maintainable feedstock of glycerol was achieved.

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