Transcriptome of porcine alveolar macrophages activated by interferon-gamma and lipopolysaccharide

文献类型： 外文期刊

第一作者： Liu, Qiang

作者： Liu, Qiang;Zhang, Yong-Li;Hu, Wei;Hu, Shou-Ping;Zhang, Zhuo;Cai, Xue-Hui;He, Xi-Jun

作者机构：

关键词： Porcine alveolar macrophages; RNA sequencing; Interferon gamma; Lipopolysaccharide; Immunoproteasome; Host restriction factor

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2018 年 503 卷 4 期

页码：

收录情况： SCI

摘要： The molecular repertoire of porcine alveolar macrophages (PAMs) is greatly affected by the microenvironment they are exposed to, and specifically by inflammatory cytokines, such as interferon gamma (IFN-gamma) released by activated lymphocytes, and microbial products, such as lipopolysaccharide (LPS). In our previous study, we found that IFN-gamma- and LPS-activated PAMs (M1) could inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication. In this study, comprehensive analysis of the expression profiles of the genes associated with the polarization of MO-type PAMs (resting) toward M1 phenotypes (activated by IFN-gamma and LPS) led to the following main results: 1) 1551 and 1823 genes were upregulated or downregulated in M1-type PAMs, respectively, compared with MO-type PAMs; 2) Among these, genes encoding ASS1 and CRTAM were the most upregulated and downregulated, respectively; 3) Genes involved in cytokine-cytokine receptor interaction and the JAK/STAT signaling pathway were significantly upregulated, suggesting their critical role in cellular activation; and 4) Genes involved in antigen proteolysis and presentation (immunoproteasome subunits), and inhibition of virus replication (host restriction factors) were significantly upregulated, emphasizing the critical role of these cytokines in immunity. Thus, our results provide important information for future studies on the role of PAM polarization in modulation of infection. (C) 2018 Elsevier Inc. All rights reserved.

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