Extraction, purification, characterization and antioxidant activities of polysaccharides from Zizyphus jujuba cv. Linzexiaozao
文献类型： 外文期刊
第一作者： Wang, Yonggang
作者： Wang, Yonggang;Xu, Ye;Liu, Xiaofeng;Yang, Mingjun;Fan, Wenguang;Ren, Haiwei;Efehi, Nehikhare;Ma, Xueqing;Efehi, Nehikhare;Wang, Xiaoli;Zhu, Xinqiang
作者机构：
关键词： Zizyphus jujuba cv. Linzexiaozao; Response surface methodology; Physicochemical characteristics; Antioxidant activity
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2018 年 118 卷
页码：
收录情况： SCI
摘要： The extraction process, purification and characterization analyses of polysaccharides (LZJP) in Ziziphus jujuba planted in Linze County, Gansu Province were investigated, respectively. The results showed a maximum polysaccharide yield of 5.72% was achieved at a solid/liquid ratio of 1:20 g/mL for 90 min at 80 degrees C. Two homogenous acidic polysaccharides (LZJP3 and LZJP4) were purified successively by DEAE-52 cellulose and Sephadex G-100 column chromatography. LZJP3 is composed of one polymer with galactose while LZJP4 is made up of two different kinds of polymers with xylose and glucose by size-exclusion chromatograph combined with multi-angle laser photometer (HPSEC-LLS) and gas chromatography (GC) analysis. LZJP3 and LZJP4 were beta-pyran polysaccharides with a large number of molecular globular aggregates by FT-IR (Fourier-transform infrared) and AFM (Atomic force microscopy) analysis, and the surface morphology exhibited smooth and filamentous staggered extension in the form of rod-like aggregation with SEM (Scanning electron microscopy) determination. Meanwhile, LZJP3 and LZJP4 exhibited antioxidant activities against DPPH, hydroxyl radical, hydrogen peroxide, superoxide radical and stronger reducing power in vitro with the concentration increasing. The results indicated that LZJPs were worthy of being developed further as a natural antioxidant in food and medicine industries. (C) 2018 Published by Elsevier B.V.
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