Development of an indirect competitive ELISA based on immunomagnetic beads' clean-up for detection of maduramicin in three chicken tissues

文献类型： 外文期刊

第一作者： Song, Meirong

作者： Song, Meirong;Xue, Yanan;Zhang, Xiya;Ding, Shuangyang;Li, Jiancheng;Xiao, Zhiming

作者机构：

关键词： Chicken; ELISA; immunomagnetic bead; maduramicin; residue

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2018 年 29 卷 1 期

页码：

收录情况： SCI

摘要： An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on immunomagnetic beads' (IMBs) clean-up was developed for detection of the residues of maduramicin (MD) in different chicken tissues. IMBs coated with a specific monoclonal antibody (MAb) against MD named MAb 2D6 were applied to enrich the residues of MD in chicken tissues. The specificities of these IMBs were well maintained and the reversibility remained at more than 73% of the original capability after being used for three times. After elution, enriched MD was detected by a conventional ic-ELISA. The limits of detection of MD were 72, 74 and 173g/kg in chicken muscle, skin and fat, and liver, respectively. Recoveries ranged from 80.0% to 115.8% with coefficients of variation being less than 11.3%. These results indicated that a rapid, robust clean-up of IMBs combining ELISA provides a simple, time-saving and environmentally friendly method to detect MD in chicken tissues.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序