Development and Evaluation of an ELISA System for Detection o PEDV-Specific IgA Antibodies in Colostrum

文献类型： 外文期刊

第一作者： Li, Ren-Feng

作者： Li, Ren-Feng;Yu, Yan;Jiang, Jin-Qing;Wang, San-Hu;Wang, Zi-Liang;Ma, Jin-You;Wang, Li-Rong;Zhang, Shou-Ping;Tian, Xiang-Qin;Yang, Yan-Yan

作者机构： Henan Inst Sci & Technol, Coll Anim Sci & Vet Med, Xinxiang 453003, Peoples R China;Xinxiang Med Coll, Med Res Ctr, Xinxiang 453003, Peoples R China;Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

关键词： ELISA; IgA antibody; Colostrum; Porcine epidemic diarrhea virus; Spike protein

期刊名称：KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI （ 2020影响因子：0.685； 五年影响因子：0.655 ）

ISSN： 1300-6045

年卷期： 2018 年 24 卷 5 期

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is a devastating swine infectious disease. Development of high-performance methods to diagnose and evaluate viral immune status remains very important to control PEDV. Here, a recombinant S1 protein-based indirect enzyme-linked immunosorbent assay (rpS1-iELISA) was developed to monitor IgA antibody in the colostrum. Optimized cut-off value of the rpS1-iELISA was determined as 0.448. Results yielded a sensitivity of 96.87% and a specificity of 100.0%. Repeatability tests indicated that the coefficients of variation of the colostrum samples within and between runs were both less than 8%.Test results of 523 field colostrum samples showed that the rpS1-IELISA had excellent agreement with immunofluorescence assay (kappa = 0.958) and better test performance than a commercial ELISA kit. This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV by measuring IgA levels in the colostrum.

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