Establishment and characterization of a telomerase-immortalized porcine bronchial epithelial cell line

文献类型： 外文期刊

第一作者： Xie, Xing

作者： Xie, Xing;Gan, Yuan;Shao, Guoqing;Zhang, Lei;Liu, Beibei;Wang, Haiyan;Feng, Yanyan;Yu, Yanfei;Chen, Rong;Wu, Meng;Zhang, Zhenzhen;Hua, Lizhong;Xiong, Qiyan;Liu, Maojun;Feng, Zhixin;Pang, Maoda;Xu, Qi;Feng, Yanyan

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关键词： cell model; hTERT-PBECs; porcine bronchial epithelial cells; porcine respiratory disease

期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）

ISSN： 0021-9541

年卷期： 2018 年 233 卷 12 期

页码：

收录情况： SCI

摘要： Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs by transfecting primary PBECs with the pEGFP-hTERT recombinant plasmid containing human telomerase reverse transcriptase (hTERT). Immortalized PBECs (hTERT-PBECs) retained the morphological and functional features of primary PBECs as indicated by cytokeratin 18 expression, telomerase activity assay, proliferation assays, karyotype analysis, and quantitative reverse-transcriptase polymerase chain reaction. Compared to primary PBECs, hTERT-PBECs had higher telomerase activity, extended replicative lifespan, and displayed enhanced proliferative activity. Moreover, this cell line is not transformed in vitro and does not exhibit a malignant phenotype in vivo, suggesting that it can be safely used in further studies. Besides, hTERT-PBECs were susceptible to swine influenza virus of H3N2 subtype and porcine circovirus type 2. In conclusion, the immortalized hTERT-PBECs represent a valuable in vitro model, which can be widely used in the study of porcine respiratory pathogenic infections.

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