Functional analysis of superoxide dismutase of Salmonella typhimurium in serum resistance and biofilm formation

文献类型： 外文期刊

第一作者： Wang, Y.

作者： Wang, Y.;Zhang, J.;Sun, L.;Wen, W.;Zhang, C.;Yi, L.;Wang, S.

作者机构：

关键词： biofilm; oxidative stress; reactive oxygen species; Salmonella enterica; superoxide dismutase

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.772； 五年影响因子：3.963 ）

ISSN： 1364-5072

年卷期： 2018 年 125 卷 5 期

页码：

收录情况： SCI

摘要： Aims Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium). Methods and ResultsConclusionsA deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase (SOD) in resistance to environmental stresses and serum, biofilm formation, epithelial cell adherence and invasion were investigated. Inactivation of sodA gene resulted in a slightly reduced growth rate, low SOD activity and increased susceptibility to reactive oxygen species and chicken serum. However, SodA was not involved in the motility of Salm. typhimurium. In addition, the sodA mutant exhibited a decreased capacity to form biofilm as well as to adhere and invade to HeLa cells, compared to the wild-type and complemented strains. Salmonella typhimurium SOD appears to play an important role in resistance to serum and oxidative stress, biofilm formation, adherence to and invasion of epithelial cells. Significance and Impact of the StudyThis study illustrates the novel roles of SodA in serum resistance and biofilm formation in Salm. typhimurium, which may provide a candidate for the biofilm eradication and prevention of infections.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序