Antibiotic resistance genes and associated bacterial communities in agricultural soils amended with different sources of animal manures

文献类型： 外文期刊

第一作者： Han, Xue-Mei

作者： Han, Xue-Mei;Yang, Li-Yuan;Li, He-Lian;Hu, Hang-Wei;Chen, Qing-Lin;Zhu, Yong-Guan;Li, Xiang-Zhen;Ma, Yi-Bing

作者机构：

关键词： Antibiotic resistance genes; Manure; Bacterial community; Mobile genetic elements; Horizontal gene transfer; Soil resistome

期刊名称：SOIL BIOLOGY & BIOCHEMISTRY （ 影响因子：7.609； 五年影响因子：8.312 ）

ISSN： 0038-0717

年卷期： 2018 年 126 卷

页码：

收录情况： SCI

摘要： Land application of animal manures is a major dissemination route for antibiotic resistance genes (ARGs) in agro-ecosystems, which are a potential risk to public health if they migrate into the food chain. Despite the fact that animal manure is a rich reservoir of ARGs our knowledge of the impacts of different types and amounts of animal manures on the temporal succession of a wide array of ARGs and potential ARG-hosts remains limited. Here, we constructed soil microcosms amended with two levels of swine, poultry or cattle manures to explore the time-course patterns of ARGs, mobile genetic elements (MGEs) including integrons and transposons, and bacterial communities. The high-throughput quantitative PCR detected 260 unique ARGs, and the application of all manure types, especially at the higher concentration (80 mg manure g(-1) soil), significantly increased the diversity and abundances of ARGs and MGEs. The abundance of ARGs in manured soils declined over time, but was still higher than that in untreated soils after 120 days, indicating the persistence of ARGs in manured soils. The next-generation sequencing revealed a clear shift in the bacterial community compositions of manured soils during the incubation. Mantel test and network analysis revealed that the ARG profile was strongly correlated with the bacterial community compositions. Variation partitioning analysis, and structure equation models, further indicated that bacterial phylogeny played a primary role in shaping the ARG profiles in manured soils. However, the significant correlation between the abundances of MGEs and ARGs suggested that the potential effects of horizontal gene transfer on the persistence of ARGs should not be overlooked. In addition, soil properties, which were strongly affected by the added manures, could also affect the ARG patterns. These findings demonstrated the temporal patterns and dissemination risk of ARGs in manured soils, which might contribute to the development of effective strategies to minimise the spread of ARGs in agro-ecosystems.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Grazing accelerates soil carbon loss by enhancing the degradability of soil water-extractable organic matter and microbial turnover in fragile grasslands

    作者：Chen, Hao;Lin, Liwen;Chen, Hao;Wang, Fei;Ji, Mukan;Kong, Weidong;Shi, Quan;Wu, Jianshuang;Wu, Jianshuang;Zhang, Xianzhou;Wang, Fei;Lin, Qimei;Zhu, Yong-Guan;Liang, Chao;Kong, Weidong;Kong, Weidong

    关键词：Grazing; Soil water-extractable organic matter; Degradability; Organic matter decomposition; Carbon loss; Grassland soil

  • Chinese milk vetch incorporation inhibits nitrification by suppressing comammox Nitrospira in subtropical paddy soils

    作者：Feng, Mengmeng;Lin, Yongxin;Ni, Xiangyin;Cheng, Yuheng;Sun, Luyuan;Liu, Jia;Hu, Hang-Wei;He, Zi-Yang;He, Ji-Zheng;Wang, Juntao;Wang, Juntao

    关键词：Chinese milk vetch; Nitrification; Nitrogen mineralization; Comammox Nitrospira; Subtropical paddy soils

  • Aspergillus Mycotoxins: The Major Food Contaminants

    作者：Xue, Mengyao;Qu, Zheng;An, Yi;Wang, Feng;Sun, Yuebing;Wang, Lili;Hou, Jie;Zhang, Chenchen;Yang, Mengmeng;Ding, Yiming;Yao, Yanpo;Moretti, Antonio;Logrieco, Antonio F.;Chu, Haiyan;Zhang, Qi;Li, Peiwu;Sun, Changpo;Ren, Xianfeng;Cui, Li;Chen, Qinglin;Li, Chengjun;Zhong, Huan;Cao, Zhiyan;Zhu, Yong-Guan

    关键词：climate change; control measures; crops; human health; mycotoxins

  • Nitrogen fertilization modulates rice phyllosphere functional genes and pathogens through fungal communities

    作者：Wu, Wei-Feng;Li, Xin-Yuan;Sun, Cheng-liang;Zhu, Yong-Guan;Lin, Xian-Yong;Chen, Song Can;Jin, Bing-Jie;Li, Gang;Jin, Bing-Jie;Li, Gang;Wu, Chun-Yan;Zhu, Yong-Guan

    关键词：Nitrogen fertilization; Phyllosphere microbiome; Functional genes; Pathogens; Bacterial -fungal interactions

  • Biogeographic patterns and drivers of soil viromes

    作者：Ma, Bin;Wang, Yiling;Zhao, Kankan;Hu, Lingfei;Xue, Ran;Dai, Hengyi;Xu, Jianming;Ma, Bin;Wang, Yiling;Zhao, Kankan;Hu, Lingfei;Xue, Ran;Dai, Hengyi;Xu, Jianming;Ma, Bin;Xue, Ran;Stirling, Erinne;Stirling, Erinne;Lv, Xiaofei;Yu, Yijun;Tang, Chao;Dong, Baiyu;Wu, Chuyi;Dahlgren, Randy A.;Tan, Xiangfeng;Zhu, Yong-Guan;Chu, Haiyan

    关键词：

  • The abundant fraction of soil microbiomes regulates the rhizosphere function in crop wild progenitors

    作者：de Celis, Miguel;Garcia-Palacios, Pablo;Fernandez-Alonso, Maria Jose;Palomino, Javier;Milla, Ruben;Fernandez-Alonso, Maria Jose;Belda, Ignacio;Garcia, Carlos;Ochoa-Hueso, Raul;Singh, Brajesh K.;Wang, Jun-Tao;Singh, Brajesh K.;Wang, Jun-Tao;Yin, Yue;Abdala-Roberts, Luis;Quijano-Medina, Teresa;Alfaro, Fernando D.;Rivera, Daniela S.;Angulo-Perez, Diego;Arthikala, Manoj-Kumar;Nanjareddy, Kalpana;Corwin, Jason;Trivedi, Pankaj;Corwin, Jason;Trivedi, Pankaj;Gui-Lan, Duan;Hernandez-Lopez, Antonio;Pasari, Babak;Shaaf, Salar;Yang, Qingwen;Zaady, Eli;Zhu, Yong-Guan;Delgado-Baquerizo, Manuel;Milla, Ruben;Garcia-Palacios, Pablo

    关键词：abundant and rare taxa; crop wild progenitors; plant domestication; plant-soil interactions; rhizosphere; soil biodiversity; soil multifunctionality

  • The abundant fraction of soil microbiomes regulates the rhizosphere function in crop wild progenitors

    作者：de Celis, Miguel;Garcia-Palacios, Pablo;Fernandez-Alonso, Maria Jose;Palomino, Javier;Milla, Ruben;Fernandez-Alonso, Maria Jose;Belda, Ignacio;Garcia, Carlos;Ochoa-Hueso, Raul;Singh, Brajesh K.;Wang, Jun-Tao;Singh, Brajesh K.;Wang, Jun-Tao;Yin, Yue;Duan, Gui-Lan;Abdala-Roberts, Luis;Quijano-Medina, Teresa;Alfaro, Fernando D.;Rivera, Daniela S.;Angulo-Perez, Diego;Arthikala, Manoj-Kumar;Nanjareddy, Kalpana;Corwin, Jason;Trivedi, Pankaj;Corwin, Jason;Trivedi, Pankaj;Hernandez-Lopez, Antonio;Pasari, Babak;Shaaf, Salar;Yang, Qingwen;Zaady, Eli;Zhu, Yong-Guan;Delgado-Baquerizo, Manuel;Milla, Ruben;Garcia-Palacios, Pablo;de Celis, Miguel;Garcia-Palacios, Pablo;Delgado-Baquerizo, Manuel;Milla, Ruben

    关键词：abundant and rare taxa; crop wild progenitors; plant domestication; plant-soil interactions; rhizosphere; soil biodiversity; soil multifunctionality

微信小程序