文献类型： 外文期刊
第一作者： Lu, Tengfei
作者： Lu, Tengfei;Pei, Wenhua;Wu, Yangnan;Guan, Weijun;Zhang, Shuang;Chen, Fenghao;Han, Xiao
作者机构：
关键词： Amniotic membrane; Mesenchymal stem cells; Sheep; Pluripotent; Differentiation
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2018 年 50 卷 5 期
页码：
收录情况： SCI
摘要： With the self-renewal capacity and the potentiality of differentiating into various cells, stem cell is a prospective source for tissue engineering. Amniotic mesenchymal stem cells (AMSCs) obtained from amnion do not evoke ethical issues, as they do with the embryonic stem cells. However, a good many experimental tissues and organs were obtained from model animals but rarely from livestock. In this study, AMSCs were obtained from aborted sheep fetuses (1-3 month) under sterile conditions. Primary AMSCs were sub-cultured to passage 25 in vitro. The gene of Oct-4, Rex-1, CD29, CD44, CD71, and CD73 were identified by RT-PCR and immunofluorescence technique. The results showed that they were positive in AMSCs. The growth of different passages cells typically appeared in S curve. Furthermore, AMSCs successfully differentiated into osteoblasts, adipocytes, and hepatocyte-like cells after procedure induction. The results showed that the AMSCs obtained from sheep demonstrated the features of multipotent stem cells. Therefore, AMSCs could be a good option for transplantation for treatment and regenerative medical projects.
分类号：
- 相关文献
作者其他论文 更多>>
-
A Novel Strategy for Constructing Ecological Index of Tea Plantations Integrating Remote Sensing and Environmental Data
作者：Mao, Yilin;Li, He;Shen, Jiazhi;Ding, Zhaotang;Sun, Litao;Wang, Yu;Xu, Yang;Fan, Kai;Han, Xiao;Ma, Qingping;Shi, Hongtao;Bi, Caihong;Feng, Yunlai
关键词：Plantations; Monitoring; Remote sensing; Temperature sensors; Temperature measurement; Ecosystems; Humidity; Convolutional neural networks gate recurrent unit (CNN-GRU); ecological tea plantation; environmental parameters; multisource remote sensing; plant community; UAV
-
Metabolic mechanism of thermal pretreatment combined with ethanol pre-fermentation to enhance anaerobic digestion of food waste: A comprehensive analysis of key enzyme-encoding genes
作者：Zhang, Shuang;Zhao, Pan;Gao, Ming;Wu, Chuanfu;Wang, Qunhui;Gao, Ming;Wu, Chuanfu;Wang, Qunhui;Sun, Xiaohong;Wang, Qunhui
关键词：Anaerobic fermentation; Pretreatment; Microbial community; Metabolic pathways; Enzyme-encoding genes
-
Resilience effects for household food expenditure and dietary diversity in rural western China
作者：Han, Xiao;Lyu, Kaiyu;Nie, Fengying;Chen, Yuquan
关键词：resilience; food expenditure; dietary diversity; China; risk management
-
Diet restriction in parents shapes the lives of their offspring: a laboratory study using a predatory mite (Acari: Phytoseiidae)
作者：Han, Xiao;Zhang, Keshi;Zhang, Zhi-Qiang;Zhang, Keshi;Zhang, Zhi-Qiang;Chen, Xia
关键词：transgenerational phenotypic plasticity; parental effect; non-genetic inheritance; Phytoseiulus persimilis; life-history traits; diet restriction
-
Nanochemistry of gold: from surface engineering to dental healthcare applications
作者：Zhang, Shuang;Wang, Zezheng;Zhang, Yangheng;Ni, Can;Li, Lingjun;Yan, Fuhua;Kong, Na;Yang, Wenrong;Kong, Na;Wang, Hongbin;Yang, Min
关键词：
-
Self-assembly of peptide nanocapsules by a solvent concentration gradient
作者：Li, Haopeng;Han, Xiao;Miserez, Ali;Yu, Jing;Li, Haopeng;Qi, Huitang;Yuan, Fenghou;Liu, Tian;Qian, Xuliang;Zou, Guijin;Gao, Huajian;Mohanram, Harini;Zou, Guijin;Gao, Huajian;Miserez, Ali;Yang, Qing;Yang, Qing;Gao, Huajian;Yu, Jing
关键词：
-
A Thermostable Cas12b-Powered Bioassay Coupled with Loop-Mediated Isothermal Amplification in a Customized "One-Pot" Vessel for Visual, Rapid, Sensitive, and On-Site Detection of Genetically Modified Crops
作者：Han, Xiao;Lu, Minghui;Zhang, Yaru;Liu, Xinru;Zhang, Qiang;Man, Shuli;Ma, Long;Zhang, Qiang;Bai, Xue;Zhao, Liangjuan
关键词：BrCas12b; genetically modified crops (GMCs); CaMV35S promoter; one-pot; portable detection; food detection
