In Vitro Culture and Biological Characteristics of Sheep Amniotic Mesenchymal Stem Cells

文献类型： 外文期刊

第一作者： Lu, Tengfei

作者： Lu, Tengfei;Pei, Wenhua;Wu, Yangnan;Guan, Weijun;Zhang, Shuang;Chen, Fenghao;Han, Xiao

作者机构：

关键词： Amniotic membrane; Mesenchymal stem cells; Sheep; Pluripotent; Differentiation

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2018 年 50 卷 5 期

页码：

收录情况： SCI

摘要： With the self-renewal capacity and the potentiality of differentiating into various cells, stem cell is a prospective source for tissue engineering. Amniotic mesenchymal stem cells (AMSCs) obtained from amnion do not evoke ethical issues, as they do with the embryonic stem cells. However, a good many experimental tissues and organs were obtained from model animals but rarely from livestock. In this study, AMSCs were obtained from aborted sheep fetuses (1-3 month) under sterile conditions. Primary AMSCs were sub-cultured to passage 25 in vitro. The gene of Oct-4, Rex-1, CD29, CD44, CD71, and CD73 were identified by RT-PCR and immunofluorescence technique. The results showed that they were positive in AMSCs. The growth of different passages cells typically appeared in S curve. Furthermore, AMSCs successfully differentiated into osteoblasts, adipocytes, and hepatocyte-like cells after procedure induction. The results showed that the AMSCs obtained from sheep demonstrated the features of multipotent stem cells. Therefore, AMSCs could be a good option for transplantation for treatment and regenerative medical projects.

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