Development of a TaqMan-based real-time PCR for detecting duck adenovirus 3

文献类型： 外文期刊

第一作者： Wan, Chunhe

作者： Wan, Chunhe;Chen, Cuiteng;Cheng, Longfei;Fu, Guanghua;Shi, Shaohua;Liu, Rongchang;Chen, Hongmei;Fu, Qiuling;Huang, Yu

作者机构：

关键词： DAdV-3; Detection; TaqMan-based; Real-time PCR assay

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2018 年 261 卷

页码：

收录情况： SCI

摘要： Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization of the qPCR conditions, the lower limit of detection for DAdV-3 infection was 40.9 copies/mu l. No cross-reactivity was observed with other duck-derived pathogens. Intra- and inter-assay variability was less than 2.30%. DAdV-3 was detected in embryos and newly hatched ducklings by qPCR assay, the findings provided evidence of possible vertical transmission of DAdV-3. The developed qPCR analysis showed high specificity, sensitivity, and reproducibility, thereby indicating that it can be used in future investigations on the pathogenesis and epidemiology of DAdV-3.

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