Identification of linear B-cell epitopes on the phosphoprotein of canine distemper virus using four monoclonal antibodies

文献类型： 外文期刊

第一作者： Li, Shuang

作者： Li, Shuang;Yi, Li;Cao, Zhigang;Cheng, Yuening;Tong, Mingwei;Wang, Jianke;Lin, Peng;Cheng, Shipeng

作者机构：

关键词： Canine distemper virus; Monoclonal antibody; Phosphoprotein; B-cell epitopes

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2018 年 257 卷

页码：

收录情况： SCI

摘要： The highly contagious canine distemper virus (CDV) is a non-segmented single-stranded negative-sense RNA virus, which belongs to the Morbillivirus genus of the Paramyxoviridae family. The phosphoprotein (P) of CDV plays the important role in the virus replication and pathogenesis. In this study, we characterized four monoclonal antibodies (MAbs), designated as Pc7, Pc8, Pc11 and Pc25 MAbs against the P protein of CDV-PS strain. A series of overlapping P protein-derived peptides representing the CDV-PS phosphoprotein (aa232-507) were screened to identify linear peptide epitopes recognized by each MAb. Finally, four epitopes, (238)SHGMGIVAG-STN249 (E2-9), (264)GPSVSAENVRQ(274) (E6-2), (390)INPELRPIIGR(400) (E27-2) and (252)TQSALKSTG(260) (E4-9), are minimal linear epitopes recognized by the Pc7, Pc8, Pc]. 1 and Pc25 MAbs, respectively. Each identified B-cell epitope was able to be recognized by CDV positive dog serum. Alignment analysis of the amino acid sequences indicated that the linear B-cell epitope of the Pc11 MAb is relatively conserved among different CDV strains, but the linear B-cell epitopes recognized by Pc7, Pc8 and Pc25 MAbs are not conserved among CDV strains. Our results revealed that the E27-2 peptide might be a common B-cell binding epitope of CDV antibodies. These findings may provide a useful basis for the development of new diagnostic assays for CDV.

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